TY - JOUR
T1 - A comparison of methods to assess cell mechanical properties
AU - Wu, Pei Hsun
AU - Aroush, Dikla Raz Ben
AU - Asnacios, Atef
AU - Chen, Wei Chiang
AU - Dokukin, Maxim E.
AU - Doss, Bryant L.
AU - Durand-Smet, Pauline
AU - Ekpenyong, Andrew
AU - Guck, Jochen
AU - Guz, Nataliia V.
AU - Janmey, Paul A.
AU - Lee, Jerry S.H.
AU - Moore, Nicole M.
AU - Ott, Albrecht
AU - Poh, Yeh Chuin
AU - Ros, Robert
AU - Sander, Mathias
AU - Sokolov, Igor
AU - Staunton, Jack R.
AU - Wang, Ning
AU - Whyte, Graeme
AU - Wirtz, Denis
N1 - Funding Information:
This research was supported by the NIH (grants U54CA143868 and R01CA174388 to D.W. and P.-H.W.; GM072744 to N.W.; GM096971 and CA193417 to P.A.J.; and CA143862 to R.R.), the NSF (grant 1510700 to R.R.), Agence Nationale de la Recherche (“ImmunoMeca” ANR-12-BSV5-0007-01, “Initiatives d’excellence” Idex ANR-11-IDEX-0005-02, and “Labex Who Am I?” ANR-11-LABX-0071 to A.A.), and the Deutsche Forschungsgemeinschaft through the collaborative research center (SFB1027 to A.O.).
PY - 2018/6/18
Y1 - 2018/6/18
N2 - The mechanical properties of cells influence their cellular and subcellular functions, including cell adhesion, migration, polarization, and differentiation, as well as organelle organization and trafficking inside the cytoplasm. Yet reported values of cell stiffness and viscosity vary substantially, which suggests differences in how the results of different methods are obtained or analyzed by different groups. To address this issue and illustrate the complementarity of certain approaches, here we present, analyze, and critically compare measurements obtained by means of some of the most widely used methods for cell mechanics: atomic force microscopy, magnetic twisting cytometry, particle-tracking microrheology, parallel-plate rheom-etry, cell monolayer rheology, and optical stretching. These measurements highlight how elastic and viscous moduli of MCF-7 breast cancer cells can vary 1,000-fold and 100-fold, respectively. We discuss the sources of these variations, including the level of applied mechanical stress, the rate of deformation, the geometry of the probe, the location probed in the cell, and the extracellular microenvironment.
AB - The mechanical properties of cells influence their cellular and subcellular functions, including cell adhesion, migration, polarization, and differentiation, as well as organelle organization and trafficking inside the cytoplasm. Yet reported values of cell stiffness and viscosity vary substantially, which suggests differences in how the results of different methods are obtained or analyzed by different groups. To address this issue and illustrate the complementarity of certain approaches, here we present, analyze, and critically compare measurements obtained by means of some of the most widely used methods for cell mechanics: atomic force microscopy, magnetic twisting cytometry, particle-tracking microrheology, parallel-plate rheom-etry, cell monolayer rheology, and optical stretching. These measurements highlight how elastic and viscous moduli of MCF-7 breast cancer cells can vary 1,000-fold and 100-fold, respectively. We discuss the sources of these variations, including the level of applied mechanical stress, the rate of deformation, the geometry of the probe, the location probed in the cell, and the extracellular microenvironment.
UR - http://www.scopus.com/inward/record.url?scp=85048699407&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048699407&partnerID=8YFLogxK
U2 - 10.1038/s41592-018-0015-1
DO - 10.1038/s41592-018-0015-1
M3 - Article
C2 - 29915189
AN - SCOPUS:85048699407
SN - 1548-7091
VL - 15
SP - 491
EP - 498
JO - Nature Methods
JF - Nature Methods
IS - 7
ER -