A comparative study of the genetic diversity of the 42 kDa fragment of the merozoite surface protein 1 in Plasmodium falciparum and P. vivax

M. Andreina Pacheco; Amanda C. Poe; William E. Collins; Altaf A. Lal; Kazuyuki Tanabe; Simon K. Kariuki; Venkatachalam Udhayakumar; Ananias A. Escalante

doi:10.1016/j.meegid.2006.08.002

A comparative study of the genetic diversity of the 42 kDa fragment of the merozoite surface protein 1 in Plasmodium falciparum and P. vivax

M. Andreina Pacheco, Amanda C. Poe, William E. Collins, Altaf A. Lal, Kazuyuki Tanabe, Simon K. Kariuki, Venkatachalam Udhayakumar, Ananias A. Escalante

Life Sciences, School of (SOLS)

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38 Scopus citations

Abstract

We investigated the genetic diversity of the 42 kDa fragment of the merozoite surface protein 1 (MSP-1) antigen in Plasmodium falciparum and P. vivax, as well as in non-human primate malarial parasites. This fragment undergoes a proteolytic cleavage generating two fragments of 19 kDa (MSP-1₁₉) and 33 kDa (MSP-1₃₃) that are critical in erythrocyte invasion. We found that overall the MSP-1₃₃ fragment exhibits greater genetic diversity than the MSP-1₁₉ regardless of the species. We have found evidence for positive natural selection only in the human malaria parasites by comparing the rate of non-synonymous versus synonymous substitutions. In addition, we found clear differences between the two major human malaria parasites. In the case of P. falciparum, positive natural selection is acting on the MSP-1₁₉ region while the MSP-1₃₃ is neutral or under purifying selection. The opposite pattern was observed in P. vivax. Our results suggest different roles of this antigen in the host-parasite immune interaction in each of the major human malarial parasites.

Original language	English (US)
Pages (from-to)	180-187
Number of pages	8
Journal	Infection, Genetics and Evolution
Volume	7
Issue number	2
DOIs	https://doi.org/10.1016/j.meegid.2006.08.002
State	Published - Mar 2007

Keywords

Genetic diversity
MSP-1
MSP-1 19 kDa
Malaria
Merozoite
Plasmodium
Vaccine

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics
Molecular Biology
Genetics
Microbiology (medical)
Infectious Diseases

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10.1016/j.meegid.2006.08.002

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@article{0bdf082d5a45490093ea724c4f319f7f,

title = "A comparative study of the genetic diversity of the 42 kDa fragment of the merozoite surface protein 1 in Plasmodium falciparum and P. vivax",

abstract = "We investigated the genetic diversity of the 42 kDa fragment of the merozoite surface protein 1 (MSP-1) antigen in Plasmodium falciparum and P. vivax, as well as in non-human primate malarial parasites. This fragment undergoes a proteolytic cleavage generating two fragments of 19 kDa (MSP-119) and 33 kDa (MSP-133) that are critical in erythrocyte invasion. We found that overall the MSP-133 fragment exhibits greater genetic diversity than the MSP-119 regardless of the species. We have found evidence for positive natural selection only in the human malaria parasites by comparing the rate of non-synonymous versus synonymous substitutions. In addition, we found clear differences between the two major human malaria parasites. In the case of P. falciparum, positive natural selection is acting on the MSP-119 region while the MSP-133 is neutral or under purifying selection. The opposite pattern was observed in P. vivax. Our results suggest different roles of this antigen in the host-parasite immune interaction in each of the major human malarial parasites.",

keywords = "Genetic diversity, MSP-1, MSP-1 19 kDa, Malaria, Merozoite, Plasmodium, Vaccine",

author = "Pacheco, {M. Andreina} and Poe, {Amanda C.} and Collins, {William E.} and Lal, {Altaf A.} and Kazuyuki Tanabe and Kariuki, {Simon K.} and Venkatachalam Udhayakumar and Escalante, {Ananias A.}",

note = "Funding Information: This research is supported by grants from the National Institutes of Health (R01 GM60740) to A.A.E. and from the Japanese Ministry of Education, Culture, Sports, Science and Technology (14021125) to K.T. We thank Andrea McCollum and two anonymous reviewers for their commentaries that improved this manuscript. The findings and conclusions in this report are those of the author(s) and do not necessarily represent the views of the Centers for Disease Control and Prevention.",

year = "2007",

month = mar,

doi = "10.1016/j.meegid.2006.08.002",

language = "English (US)",

volume = "7",

pages = "180--187",

journal = "Infection, Genetics and Evolution",

issn = "1567-1348",

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AU - Pacheco, M. Andreina

AU - Poe, Amanda C.

AU - Collins, William E.

AU - Lal, Altaf A.

AU - Tanabe, Kazuyuki

AU - Kariuki, Simon K.

AU - Udhayakumar, Venkatachalam

AU - Escalante, Ananias A.

N1 - Funding Information: This research is supported by grants from the National Institutes of Health (R01 GM60740) to A.A.E. and from the Japanese Ministry of Education, Culture, Sports, Science and Technology (14021125) to K.T. We thank Andrea McCollum and two anonymous reviewers for their commentaries that improved this manuscript. The findings and conclusions in this report are those of the author(s) and do not necessarily represent the views of the Centers for Disease Control and Prevention.

PY - 2007/3

Y1 - 2007/3

N2 - We investigated the genetic diversity of the 42 kDa fragment of the merozoite surface protein 1 (MSP-1) antigen in Plasmodium falciparum and P. vivax, as well as in non-human primate malarial parasites. This fragment undergoes a proteolytic cleavage generating two fragments of 19 kDa (MSP-119) and 33 kDa (MSP-133) that are critical in erythrocyte invasion. We found that overall the MSP-133 fragment exhibits greater genetic diversity than the MSP-119 regardless of the species. We have found evidence for positive natural selection only in the human malaria parasites by comparing the rate of non-synonymous versus synonymous substitutions. In addition, we found clear differences between the two major human malaria parasites. In the case of P. falciparum, positive natural selection is acting on the MSP-119 region while the MSP-133 is neutral or under purifying selection. The opposite pattern was observed in P. vivax. Our results suggest different roles of this antigen in the host-parasite immune interaction in each of the major human malarial parasites.

AB - We investigated the genetic diversity of the 42 kDa fragment of the merozoite surface protein 1 (MSP-1) antigen in Plasmodium falciparum and P. vivax, as well as in non-human primate malarial parasites. This fragment undergoes a proteolytic cleavage generating two fragments of 19 kDa (MSP-119) and 33 kDa (MSP-133) that are critical in erythrocyte invasion. We found that overall the MSP-133 fragment exhibits greater genetic diversity than the MSP-119 regardless of the species. We have found evidence for positive natural selection only in the human malaria parasites by comparing the rate of non-synonymous versus synonymous substitutions. In addition, we found clear differences between the two major human malaria parasites. In the case of P. falciparum, positive natural selection is acting on the MSP-119 region while the MSP-133 is neutral or under purifying selection. The opposite pattern was observed in P. vivax. Our results suggest different roles of this antigen in the host-parasite immune interaction in each of the major human malarial parasites.

KW - Genetic diversity

KW - MSP-1

KW - MSP-1 19 kDa

KW - Malaria

KW - Merozoite

KW - Plasmodium

KW - Vaccine

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A comparative study of the genetic diversity of the 42 kDa fragment of the merozoite surface protein 1 in Plasmodium falciparum and P. vivax

Abstract

Keywords

ASJC Scopus subject areas

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