TY - JOUR
T1 - A combination vaccine confers full protection against co-infections with influenza, herpes simplex and respiratory syncytial viruses
AU - Talaat, Adel M.
AU - Lyons, Rick
AU - Johnston, Stephen Albert
N1 - Funding Information:
We wish to thank Kathryn Sykes, Katherine Stemke-Hale, Michele Lee for technical support and Mike McGuire, Linda Hynan for helpful discussions. This work was supported by a grant from DARPA to SAJ. AMT is supported in part by a Cardiology Training Grant fellowship.
PY - 2001/11/12
Y1 - 2001/11/12
N2 - Combined/composite vaccines should be useful in reducing the number of vaccinations and provide more flexibility in confronting biological warfare scenarios. We tested the effectiveness of a composite genetic vaccine designed from previously known protective antigens directed against influenza A virus (INF-A), herpes simplex virus type-1 (HSV-1) and respiratory syncytial virus (RSV) in a mouse-based challenge. Immunizing mice with a pool of four plasmids; INF-A haemagglutinin (HA), INF-A nucleoprotein (NP), HSV-1 glycoprotein D (gD) and RSV glycoprotein F, against the three pathogens provided full protection when mice were challenged with each pathogen. Remarkably, mice challenged with all three pathogens at once were also fully protected, even when a bacterial pathogen, Mycoplasma pulmonis, was included in the challenge. If these results are extendable to other combinations of vaccines in other hosts, it would support the development of gene vaccines as multi-component, combination vaccines.
AB - Combined/composite vaccines should be useful in reducing the number of vaccinations and provide more flexibility in confronting biological warfare scenarios. We tested the effectiveness of a composite genetic vaccine designed from previously known protective antigens directed against influenza A virus (INF-A), herpes simplex virus type-1 (HSV-1) and respiratory syncytial virus (RSV) in a mouse-based challenge. Immunizing mice with a pool of four plasmids; INF-A haemagglutinin (HA), INF-A nucleoprotein (NP), HSV-1 glycoprotein D (gD) and RSV glycoprotein F, against the three pathogens provided full protection when mice were challenged with each pathogen. Remarkably, mice challenged with all three pathogens at once were also fully protected, even when a bacterial pathogen, Mycoplasma pulmonis, was included in the challenge. If these results are extendable to other combinations of vaccines in other hosts, it would support the development of gene vaccines as multi-component, combination vaccines.
KW - Combination/composite vaccine
KW - HSV
KW - Influenza A
KW - RSV
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U2 - 10.1016/S0264-410X(01)00352-8
DO - 10.1016/S0264-410X(01)00352-8
M3 - Article
C2 - 11672920
AN - SCOPUS:0035851350
SN - 0264-410X
VL - 20
SP - 538
EP - 544
JO - Vaccine
JF - Vaccine
IS - 3-4
ER -