A collection of tri- and tetranucleotide repeat markers used to generate high quality, high resolution human genome-wide linkage maps

Val C. Sheffield, James L. Weber, Kenneth Buetow, Jeffrey C. Murray, Dee Ann Even, Kerry Wiles, Julie M. Gastier, Jacqueline C. Pulido, Chandri Yandava, Sara L. Sunden, Gretel Mattes, Thomas Businga, Anne Mcclain, John Beck, Titia Scherpler, John Gilliam, John Zhong, Geoffrey M. Duyk

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

We report a collection of tri- and tetranucleotide repeat sequence polymorphic markers used to construct genome-wide human linkage maps. Using a strategy of marker selection to create libraries highly enriched for the presence of specific tandem repeat elements, we have developed over 2000 high heterozygosity, easy-to-use tn- and tetranucieotide short tandem repeat polymorphisms (STRPs). To date, over 1300 of these markers have been genotyped on the CEPH reference families. Additional STRPs were assigned to chromosomes using human monochromosomal somatic cell hybrids. The linkage maps constructed with these markers have been integrated with other CEPH genotypes into a comprehensive high density linkage map. These STRPs have been shown to be robust for genotyping in a variety of laboratories using a variety of methods. The high quality of these STRPs makes them ideal candidates for use in genome-wide linkage searches. The integration of these markers with physical mapping reagents and other genetic markers will create a resource for moving from genome-wide linkage searches to rapid subiocalization of disease loci.

Original languageEnglish (US)
Pages (from-to)1837-1844
Number of pages8
JournalHuman Molecular Genetics
Volume4
Issue number10
DOIs
StatePublished - Oct 1995
Externally publishedYes

Fingerprint

Trinucleotide Repeats
Human Genome
Polymorphism
Microsatellite Repeats
Linkage
Genome
High Resolution
Genes
Chromosomes
Tandem Repeat Sequences
Hybrid Cells
Human Chromosomes
Genotype
Genetic Markers
Libraries
Chromosome
Locus
Human
Resources
Cell

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Molecular Biology
  • Genetics(clinical)
  • Genetics

Cite this

A collection of tri- and tetranucleotide repeat markers used to generate high quality, high resolution human genome-wide linkage maps. / Sheffield, Val C.; Weber, James L.; Buetow, Kenneth; Murray, Jeffrey C.; Even, Dee Ann; Wiles, Kerry; Gastier, Julie M.; Pulido, Jacqueline C.; Yandava, Chandri; Sunden, Sara L.; Mattes, Gretel; Businga, Thomas; Mcclain, Anne; Beck, John; Scherpler, Titia; Gilliam, John; Zhong, John; Duyk, Geoffrey M.

In: Human Molecular Genetics, Vol. 4, No. 10, 10.1995, p. 1837-1844.

Research output: Contribution to journalArticle

Sheffield, VC, Weber, JL, Buetow, K, Murray, JC, Even, DA, Wiles, K, Gastier, JM, Pulido, JC, Yandava, C, Sunden, SL, Mattes, G, Businga, T, Mcclain, A, Beck, J, Scherpler, T, Gilliam, J, Zhong, J & Duyk, GM 1995, 'A collection of tri- and tetranucleotide repeat markers used to generate high quality, high resolution human genome-wide linkage maps', Human Molecular Genetics, vol. 4, no. 10, pp. 1837-1844. https://doi.org/10.1093/hmg/4.10.1837
Sheffield, Val C. ; Weber, James L. ; Buetow, Kenneth ; Murray, Jeffrey C. ; Even, Dee Ann ; Wiles, Kerry ; Gastier, Julie M. ; Pulido, Jacqueline C. ; Yandava, Chandri ; Sunden, Sara L. ; Mattes, Gretel ; Businga, Thomas ; Mcclain, Anne ; Beck, John ; Scherpler, Titia ; Gilliam, John ; Zhong, John ; Duyk, Geoffrey M. / A collection of tri- and tetranucleotide repeat markers used to generate high quality, high resolution human genome-wide linkage maps. In: Human Molecular Genetics. 1995 ; Vol. 4, No. 10. pp. 1837-1844.
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