A collection of tri- and tetranucleotide repeat markers used to generate high quality, high resolution human genome-wide linkage maps

Val C. Sheffield; James L. Weber; Kenneth H. Buetow; Jeffrey C. Murray; Dee Ann Even; Kerry Wiles; Julie M. Gastier; Jacqueline C. Pulido; Chandri Yandava; Sara L. Sunden; Gretel Mattes; Thomas Businga; Anne Mcclain; John Beck; Titia Scherpler; John Gilliam; John Zhong; Geoffrey M. Duyk

doi:10.1093/hmg/4.10.1837

A collection of tri- and tetranucleotide repeat markers used to generate high quality, high resolution human genome-wide linkage maps

Val C. Sheffield, James L. Weber, Kenneth H. Buetow, Jeffrey C. Murray, Dee Ann Even, Kerry Wiles, Julie M. Gastier, Jacqueline C. Pulido, Chandri Yandava, Sara L. Sunden, Gretel Mattes, Thomas Businga, Anne Mcclain, John Beck, Titia Scherpler, John Gilliam, John Zhong, Geoffrey M. Duyk

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We report a collection of tri- and tetranucleotide repeat sequence polymorphic markers used to construct genome-wide human linkage maps. Using a strategy of marker selection to create libraries highly enriched for the presence of specific tandem repeat elements, we have developed over 2000 high heterozygosity, easy-to-use tn- and tetranucieotide short tandem repeat polymorphisms (STRPs). To date, over 1300 of these markers have been genotyped on the CEPH reference families. Additional STRPs were assigned to chromosomes using human monochromosomal somatic cell hybrids. The linkage maps constructed with these markers have been integrated with other CEPH genotypes into a comprehensive high density linkage map. These STRPs have been shown to be robust for genotyping in a variety of laboratories using a variety of methods. The high quality of these STRPs makes them ideal candidates for use in genome-wide linkage searches. The integration of these markers with physical mapping reagents and other genetic markers will create a resource for moving from genome-wide linkage searches to rapid subiocalization of disease loci.

Original language	English (US)
Pages (from-to)	1837-1844
Number of pages	8
Journal	Human Molecular Genetics
Volume	4
Issue number	10
DOIs	https://doi.org/10.1093/hmg/4.10.1837
State	Published - Oct 1995
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics
Genetics(clinical)

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10.1093/hmg/4.10.1837

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Sheffield, V. C., Weber, J. L., Buetow, K. H., Murray, J. C., Even, D. A., Wiles, K., Gastier, J. M., Pulido, J. C., Yandava, C., Sunden, S. L., Mattes, G., Businga, T., Mcclain, A., Beck, J., Scherpler, T., Gilliam, J., Zhong, J., & Duyk, G. M. (1995). A collection of tri- and tetranucleotide repeat markers used to generate high quality, high resolution human genome-wide linkage maps. Human Molecular Genetics, 4(10), 1837-1844. https://doi.org/10.1093/hmg/4.10.1837

Sheffield, VC, Weber, JL, Buetow, KH, Murray, JC, Even, DA, Wiles, K, Gastier, JM, Pulido, JC, Yandava, C, Sunden, SL, Mattes, G, Businga, T, Mcclain, A, Beck, J, Scherpler, T, Gilliam, J, Zhong, J & Duyk, GM 1995, 'A collection of tri- and tetranucleotide repeat markers used to generate high quality, high resolution human genome-wide linkage maps', Human Molecular Genetics, vol. 4, no. 10, pp. 1837-1844. https://doi.org/10.1093/hmg/4.10.1837

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title = "A collection of tri- and tetranucleotide repeat markers used to generate high quality, high resolution human genome-wide linkage maps",

abstract = "We report a collection of tri- and tetranucleotide repeat sequence polymorphic markers used to construct genome-wide human linkage maps. Using a strategy of marker selection to create libraries highly enriched for the presence of specific tandem repeat elements, we have developed over 2000 high heterozygosity, easy-to-use tn- and tetranucieotide short tandem repeat polymorphisms (STRPs). To date, over 1300 of these markers have been genotyped on the CEPH reference families. Additional STRPs were assigned to chromosomes using human monochromosomal somatic cell hybrids. The linkage maps constructed with these markers have been integrated with other CEPH genotypes into a comprehensive high density linkage map. These STRPs have been shown to be robust for genotyping in a variety of laboratories using a variety of methods. The high quality of these STRPs makes them ideal candidates for use in genome-wide linkage searches. The integration of these markers with physical mapping reagents and other genetic markers will create a resource for moving from genome-wide linkage searches to rapid subiocalization of disease loci.",

author = "Sheffield, {Val C.} and Weber, {James L.} and Buetow, {Kenneth H.} and Murray, {Jeffrey C.} and Even, {Dee Ann} and Kerry Wiles and Gastier, {Julie M.} and Pulido, {Jacqueline C.} and Chandri Yandava and Sunden, {Sara L.} and Gretel Mattes and Thomas Businga and Anne Mcclain and John Beck and Titia Scherpler and John Gilliam and John Zhong and Duyk, {Geoffrey M.}",

note = "Funding Information: We would like to thank the following individuals for excellent technical assistance, without whom this work would not have been possible: Randal Anderson, Thomas Brody, Wei Cao, Donna David, Carla Franzen, Jacqueline Ghazizadah, Susan Ludwigsen, Frank Manion, Janice Miller, Brian Morrison, Nancy Newkirk, John Poulter, John Quillen, Ray Reichert, Tanya Rokhlina, Steve Rosen, Sherry Satzman, Matt Stephenson, Bob Stodola, Michelle Vanderlit, Dawn Wiegel and Molly Wise. The integration of the markers and maps reported here was made possible by the availability of a common set of refererence families from CEPH. This work was supported by NIH grant p50HGOO835.",

year = "1995",

month = oct,

doi = "10.1093/hmg/4.10.1837",

language = "English (US)",

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T1 - A collection of tri- and tetranucleotide repeat markers used to generate high quality, high resolution human genome-wide linkage maps

AU - Sheffield, Val C.

AU - Weber, James L.

AU - Buetow, Kenneth H.

AU - Murray, Jeffrey C.

AU - Even, Dee Ann

AU - Wiles, Kerry

AU - Gastier, Julie M.

AU - Pulido, Jacqueline C.

AU - Yandava, Chandri

AU - Sunden, Sara L.

AU - Mattes, Gretel

AU - Businga, Thomas

AU - Mcclain, Anne

AU - Beck, John

AU - Scherpler, Titia

AU - Gilliam, John

AU - Zhong, John

AU - Duyk, Geoffrey M.

N1 - Funding Information: We would like to thank the following individuals for excellent technical assistance, without whom this work would not have been possible: Randal Anderson, Thomas Brody, Wei Cao, Donna David, Carla Franzen, Jacqueline Ghazizadah, Susan Ludwigsen, Frank Manion, Janice Miller, Brian Morrison, Nancy Newkirk, John Poulter, John Quillen, Ray Reichert, Tanya Rokhlina, Steve Rosen, Sherry Satzman, Matt Stephenson, Bob Stodola, Michelle Vanderlit, Dawn Wiegel and Molly Wise. The integration of the markers and maps reported here was made possible by the availability of a common set of refererence families from CEPH. This work was supported by NIH grant p50HGOO835.

PY - 1995/10

Y1 - 1995/10

N2 - We report a collection of tri- and tetranucleotide repeat sequence polymorphic markers used to construct genome-wide human linkage maps. Using a strategy of marker selection to create libraries highly enriched for the presence of specific tandem repeat elements, we have developed over 2000 high heterozygosity, easy-to-use tn- and tetranucieotide short tandem repeat polymorphisms (STRPs). To date, over 1300 of these markers have been genotyped on the CEPH reference families. Additional STRPs were assigned to chromosomes using human monochromosomal somatic cell hybrids. The linkage maps constructed with these markers have been integrated with other CEPH genotypes into a comprehensive high density linkage map. These STRPs have been shown to be robust for genotyping in a variety of laboratories using a variety of methods. The high quality of these STRPs makes them ideal candidates for use in genome-wide linkage searches. The integration of these markers with physical mapping reagents and other genetic markers will create a resource for moving from genome-wide linkage searches to rapid subiocalization of disease loci.

AB - We report a collection of tri- and tetranucleotide repeat sequence polymorphic markers used to construct genome-wide human linkage maps. Using a strategy of marker selection to create libraries highly enriched for the presence of specific tandem repeat elements, we have developed over 2000 high heterozygosity, easy-to-use tn- and tetranucieotide short tandem repeat polymorphisms (STRPs). To date, over 1300 of these markers have been genotyped on the CEPH reference families. Additional STRPs were assigned to chromosomes using human monochromosomal somatic cell hybrids. The linkage maps constructed with these markers have been integrated with other CEPH genotypes into a comprehensive high density linkage map. These STRPs have been shown to be robust for genotyping in a variety of laboratories using a variety of methods. The high quality of these STRPs makes them ideal candidates for use in genome-wide linkage searches. The integration of these markers with physical mapping reagents and other genetic markers will create a resource for moving from genome-wide linkage searches to rapid subiocalization of disease loci.

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A collection of tri- and tetranucleotide repeat markers used to generate high quality, high resolution human genome-wide linkage maps

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