A chemifluorescent immunoassay for the determination of marinobufagenin in body fluids

Daad Abi-Ghanem, Xinzhong Lai, Luc R. Berghman, Darijana Horvat, Jing Li, Daniel Romo, Mohammad N. Uddin, Yoshiaki Kamano, Toshihiko Nogawa, Jun-Ping Xu, George Pettit, Jules B. Puschett

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We describe here the development of a chemifluorescent competitive enzyme-linked immunosorbent assay (ELISA) that quantifies marinobufagenin (MBG) levels in biological fluids. Based on a polyclonal antibody raised against a novel MBG-bovine serum albumin conjugate, this assay achieved an MBG detection limit of less than 9pg/mL. MBG levels in various rat urine and serum samples were effectively determined using this methodology. Interassay variability averaged 9.8%, while intra-assay variability averaged 1.9 and 2.5% in representative serum and urine samples, respectively. Recovery of exogenously added MBG averaged 106%, and parallelism data further established the accuracy of the assay. Employment of this assay to detect MBG abnormalities represents a powerful tool for the possible diagnosis, prevention and management of human hypertensive states, particularly preeclampsia.

Original languageEnglish (US)
Pages (from-to)31-46
Number of pages16
JournalJournal of Immunoassay and Immunochemistry
Volume32
Issue number1
DOIs
StatePublished - Jan 2011

Fingerprint

Body fluids
Body Fluids
Immunoassay
Assays
Urine
Immunosorbents
Bovine Serum Albumin
Pre-Eclampsia
Serum
Limit of Detection
marinobufagenin
Rats
Enzyme-Linked Immunosorbent Assay
Recovery
Fluids
Antibodies
Enzymes

Keywords

  • bufodienolides
  • cardiac glycosides
  • diagnostic
  • hypertension
  • immunoassay
  • marinobufagenin

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Biochemistry
  • Medical Laboratory Technology

Cite this

Abi-Ghanem, D., Lai, X., Berghman, L. R., Horvat, D., Li, J., Romo, D., ... Puschett, J. B. (2011). A chemifluorescent immunoassay for the determination of marinobufagenin in body fluids. Journal of Immunoassay and Immunochemistry, 32(1), 31-46. https://doi.org/10.1080/15321819.2010.538107

A chemifluorescent immunoassay for the determination of marinobufagenin in body fluids. / Abi-Ghanem, Daad; Lai, Xinzhong; Berghman, Luc R.; Horvat, Darijana; Li, Jing; Romo, Daniel; Uddin, Mohammad N.; Kamano, Yoshiaki; Nogawa, Toshihiko; Xu, Jun-Ping; Pettit, George; Puschett, Jules B.

In: Journal of Immunoassay and Immunochemistry, Vol. 32, No. 1, 01.2011, p. 31-46.

Research output: Contribution to journalArticle

Abi-Ghanem, D, Lai, X, Berghman, LR, Horvat, D, Li, J, Romo, D, Uddin, MN, Kamano, Y, Nogawa, T, Xu, J-P, Pettit, G & Puschett, JB 2011, 'A chemifluorescent immunoassay for the determination of marinobufagenin in body fluids', Journal of Immunoassay and Immunochemistry, vol. 32, no. 1, pp. 31-46. https://doi.org/10.1080/15321819.2010.538107
Abi-Ghanem, Daad ; Lai, Xinzhong ; Berghman, Luc R. ; Horvat, Darijana ; Li, Jing ; Romo, Daniel ; Uddin, Mohammad N. ; Kamano, Yoshiaki ; Nogawa, Toshihiko ; Xu, Jun-Ping ; Pettit, George ; Puschett, Jules B. / A chemifluorescent immunoassay for the determination of marinobufagenin in body fluids. In: Journal of Immunoassay and Immunochemistry. 2011 ; Vol. 32, No. 1. pp. 31-46.
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