A chemical synthesis of adenosine 5' [γ 32P]triphosphate

Sidney Hecht, J. W. Kozarich

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Adenosine 5' [γ 32P]triphosphate has been prepared from adenosine 5' diphosphate by condensation of the phosphorimidazolidate of ADP with 32Pi. No purification of intermediates is required in this efficient synthesis, which is convenient for micro scale conversions. This procedure permits the preparation of [γ 32P]ATP of high specific activity and should be applicable to the synthesis of many nucleoside triphosphates, including some which may not be accessible by enzymatic means.

Original languageEnglish (US)
Pages (from-to)307-309
Number of pages3
JournalBiochimica et Biophysica Acta
Volume33
Issue number3
StatePublished - 1973
Externally publishedYes

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Adenosine
Adenosine Diphosphate
Nucleosides
Adenosine Triphosphate
triphosphoric acid

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A chemical synthesis of adenosine 5' [γ 32P]triphosphate. / Hecht, Sidney; Kozarich, J. W.

In: Biochimica et Biophysica Acta, Vol. 33, No. 3, 1973, p. 307-309.

Research output: Contribution to journalArticle

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