Adenosine 5′-[γ-32P]triphosphate has been prepared from adenosine 5′-diphosphate by condensation of the phosphorimidazolidate of ADP with 32Pi. No purification of intermediates is required in this efficient synthesis, which is convenient for micro-scale conversions. This procedure permits the preparation of [γ-32P]ATP of high specific activity and should be applicable to the synthesis of many nucleoside triphosphates, including some which may not be accessible by enzymatic means.
|Original language||English (US)|
|Number of pages||3|
|Journal||BBA Section Nucleic Acids And Protein Synthesis|
|State||Published - Dec 21 1973|
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