A chemical synthesis of adenosine 5′-[γ-32P]triphosphate

Sidney Hecht, John W. Kozarich

Research output: Contribution to journalArticle

Abstract

Adenosine 5′-[γ-32P]triphosphate has been prepared from adenosine 5′-diphosphate by condensation of the phosphorimidazolidate of ADP with 32Pi. No purification of intermediates is required in this efficient synthesis, which is convenient for micro-scale conversions. This procedure permits the preparation of [γ-32P]ATP of high specific activity and should be applicable to the synthesis of many nucleoside triphosphates, including some which may not be accessible by enzymatic means.

Original languageEnglish (US)
Pages (from-to)307-309
Number of pages3
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume331
Issue number3
DOIs
StatePublished - Dec 21 1973
Externally publishedYes

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Adenosine
Diphosphates
Nucleosides
Adenosine Diphosphate
Purification
Condensation
Adenosine Triphosphate
triphosphoric acid

Cite this

A chemical synthesis of adenosine 5′-[γ-32P]triphosphate. / Hecht, Sidney; Kozarich, John W.

In: BBA Section Nucleic Acids And Protein Synthesis, Vol. 331, No. 3, 21.12.1973, p. 307-309.

Research output: Contribution to journalArticle

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