A chemical synthesis of adenosine 5′-[γ-32P]triphosphate

Sidney M. Hecht; John W. Kozarich

doi:10.1016/0005-2787(73)90015-4

A chemical synthesis of adenosine 5′-[γ-³²P]triphosphate

Sidney M. Hecht, John W. Kozarich

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Adenosine 5′-[γ-³²P]triphosphate has been prepared from adenosine 5′-diphosphate by condensation of the phosphorimidazolidate of ADP with ³²P_i. No purification of intermediates is required in this efficient synthesis, which is convenient for micro-scale conversions. This procedure permits the preparation of [γ-³²P]ATP of high specific activity and should be applicable to the synthesis of many nucleoside triphosphates, including some which may not be accessible by enzymatic means.

Original language	English (US)
Pages (from-to)	307-309
Number of pages	3
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	331
Issue number	3
DOIs	https://doi.org/10.1016/0005-2787(73)90015-4
State	Published - Dec 21 1973
Externally published	Yes

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General Medicine

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title = "A chemical synthesis of adenosine 5′-[γ-32P]triphosphate",

abstract = "Adenosine 5′-[γ-32P]triphosphate has been prepared from adenosine 5′-diphosphate by condensation of the phosphorimidazolidate of ADP with 32Pi. No purification of intermediates is required in this efficient synthesis, which is convenient for micro-scale conversions. This procedure permits the preparation of [γ-32P]ATP of high specific activity and should be applicable to the synthesis of many nucleoside triphosphates, including some which may not be accessible by enzymatic means.",

author = "Hecht, {Sidney M.} and Kozarich, {John W.}",

note = "Funding Information: Acknowledgment is made to the donors of the Petroleum Research Fund, administered by the A.C.S., for financial support of this research.",

year = "1973",

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doi = "10.1016/0005-2787(73)90015-4",

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T1 - A chemical synthesis of adenosine 5′-[γ-32P]triphosphate

AU - Hecht, Sidney M.

AU - Kozarich, John W.

N1 - Funding Information: Acknowledgment is made to the donors of the Petroleum Research Fund, administered by the A.C.S., for financial support of this research.

PY - 1973/12/21

Y1 - 1973/12/21

N2 - Adenosine 5′-[γ-32P]triphosphate has been prepared from adenosine 5′-diphosphate by condensation of the phosphorimidazolidate of ADP with 32Pi. No purification of intermediates is required in this efficient synthesis, which is convenient for micro-scale conversions. This procedure permits the preparation of [γ-32P]ATP of high specific activity and should be applicable to the synthesis of many nucleoside triphosphates, including some which may not be accessible by enzymatic means.

AB - Adenosine 5′-[γ-32P]triphosphate has been prepared from adenosine 5′-diphosphate by condensation of the phosphorimidazolidate of ADP with 32Pi. No purification of intermediates is required in this efficient synthesis, which is convenient for micro-scale conversions. This procedure permits the preparation of [γ-32P]ATP of high specific activity and should be applicable to the synthesis of many nucleoside triphosphates, including some which may not be accessible by enzymatic means.

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ER -

A chemical synthesis of adenosine 5′-[γ-³²P]triphosphate

Abstract

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