A central role for transcription factor C/EBP-β in regulating CD1d gene expression in human keratinocytes

Hashmat Sikder, Yuming Zhao, Anna Balato, Andre Chapoval, Rita Fishelevich, Padmaja Gade, Ishwar S. Singh, Dhananjaya V. Kalvakolanu, Peter F. Johnson, Anthony A. Gaspari

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9 Scopus citations

Abstract

CD1d is a nonclassical Ag-presenting molecule that presents glycolipid Ags to NKT cells that are involved in immune defense and tumor rejection. It also plays a role in immunoregulatory functions in the epidermis. The mechanisms controlling the expression of CD1d are not well understood. Therefore, we cloned the CD1d gene promoter and characterized its activities in primary human keratinocytes and other cell lines of epithelial origin. We found that a CCAAT box in the CD1d promoter is required for its expression in keratinocytes. We show here that transcription factor C/EBP-β binds to the CCAAT box in the CD1d promoter in vitro and in vivo. Consistent with these observations, deletion of the gene encoding for C/EBP-βcaused a loss of CD1d expression. The in vivo regulation of CD1d has significant implications for the pathologic mechanisms of certain immunologic skin diseases in which NKT cells play a role, such as allergic contact dermatitis and psoriasis. Together, these data show a central role for C/EBP-β in regulating CD1d transcription.

Original languageEnglish (US)
Pages (from-to)1657-1666
Number of pages10
JournalJournal of Immunology
Volume183
Issue number3
DOIs
StatePublished - Aug 1 2009
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Sikder, H., Zhao, Y., Balato, A., Chapoval, A., Fishelevich, R., Gade, P., Singh, I. S., Kalvakolanu, D. V., Johnson, P. F., & Gaspari, A. A. (2009). A central role for transcription factor C/EBP-β in regulating CD1d gene expression in human keratinocytes. Journal of Immunology, 183(3), 1657-1666. https://doi.org/10.4049/jimmunol.0900057