A cellular isolation system for real-time single-cell oxygen consumption monitoring

Joe Dragavon, Tim Molter, Cody Young, Tim Strovas, Sarah McQuaide, Mark Holl, Meng Zhang, Brad Cookson, Alex Jen, Mary Lidstrom, Deirdre Meldrum, Lloyd Burgess

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

The development of a cellular isolation system (CIS) that enables the monitoring of singlecell oxygen consumption rates in real time is presented. The CIS was developed through a multidisciplinary effort within the Microscale Life Sciences Center (MLSC) at the University of Washington. The system comprises arrays of microwells containing Pt-porphyrinembedded polystyrene microspheres as the reporter chemistry, a lid actuator system and a gated intensified imaging camera, all mounted on a temperature-stabilized confocal microscope platform. Oxygen consumption determination experiments were performed on RAW264.7 mouse macrophage cells as proof of principle. Repeatable and consistent measurements indicate that the oxygen measurements did not adversely affect the physiological state of the cells measured. The observation of physiological rates in real time allows studies of cell-to-cell heterogeneity in oxygen consumption rate to be performed. Such studies have implications in understanding the role of mitochondrial function in the progression of inflammatory-based diseases, and in diagnosing and treating such diseases.

Original languageEnglish (US)
Pages (from-to)S151-S159
JournalJournal of the Royal Society Interface
Volume5
Issue numberSUPPL.2
DOIs
StatePublished - Jan 1 2008

Keywords

  • Mouse macrophage
  • Respiration rate
  • Single-cell analysis

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering

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    Dragavon, J., Molter, T., Young, C., Strovas, T., McQuaide, S., Holl, M., Zhang, M., Cookson, B., Jen, A., Lidstrom, M., Meldrum, D., & Burgess, L. (2008). A cellular isolation system for real-time single-cell oxygen consumption monitoring. Journal of the Royal Society Interface, 5(SUPPL.2), S151-S159. https://doi.org/10.1098/rsif.2008.0106.focus