A cell-based immunobiosensor with engineered molecular recognition - Part III

Engineering molecular recognition

Darren L. Page, Vincent Pizziconi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have been studying the feasibility of exploiting the recognition and amplification abilities of living immune cells for the development of hybrid immunosensors. Our group has previously reported that cell metabolic activation responses, induced by calcium ionophore A23187, can be directly transduced using calorimetric transducers, and that enzyme systems can be integrated to enhance sensing response time and output. In this study our goal was to determine the feasibility of transducing the thermal activation responses of mast cells molecularly engineered to a specific antigen. Rat peritoneal mast cells were sensitized to the model antigenic analyte dinitrophenylated-albumin (DNPA), with monoclonal anti-DNP-A IgE, and challenged with antigen at final concentrations of 10 or 100 ng/ml. The addition of antigen resulted in the molecular triggering of cell activation, yielding thermal responses similar to those obtained previously with the ionophore model. A peak thermal response of 1.7 μW/5 x 105 cells was obtained within approximately 7 min of addition of antigen. The incorporation of selected amplification enzyme systems increased peak thermal outputs approximately three-fold, and reduced peak thermal response times to less than 3 min. A preliminary regression analysis of these data suggests a quantitative relationship exists between analyte concentration and peak thermal response (R = 0.988). These results support the feasibility and potential versatility of cell-based immunobiosensors for the selective detection and quantification of immunological analytes of interest.

Original languageEnglish (US)
Pages (from-to)559-566
Number of pages8
JournalBiosensors and Bioelectronics
Volume12
Issue number7
DOIs
StatePublished - 1997

Fingerprint

Molecular recognition
Hot Temperature
Antigens
Ionophores
Chemical activation
Mast Cells
Reaction Time
Amplification
Enzymes
Immunosensors
Aptitude
Calcium Ionophores
Hybrid Cells
Calcimycin
Transducers
Regression analysis
Immunoglobulin E
Rats
Albumins
Calcium

Keywords

  • Antibody receptors
  • Antigenic analyte
  • Immune cells
  • Immunodiagnostics
  • Immunosensor
  • Mast cells
  • Microcalorimetry
  • Thermoelectric
  • Whole cell

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

A cell-based immunobiosensor with engineered molecular recognition - Part III : Engineering molecular recognition. / Page, Darren L.; Pizziconi, Vincent.

In: Biosensors and Bioelectronics, Vol. 12, No. 7, 1997, p. 559-566.

Research output: Contribution to journalArticle

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