A bodian method for mounted frozen sections

Barbara Evelyn Herr, Paul D. Coleman, Robert C. Griggs

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

For application of the Bodian method to frozen sections, cut frozen peripheral nerve or muscle at 10μm and mount. Fix for 4 days in 18% parts 80% ethanol, 1 part 10% formalin, and 1 part glacial acetic acid. Fix central nervous tissue in the same mixture prior to freezing and sectioning, and after mounting postfix for 4 days. Impregnate by the Bodian procedure. The results equal Bodian stains of paraffin sections. The technique is simple and reliable. The use of 10μm frozen sections produces little artifact and allows alternate serial sections to be stained with other techniques.

Original languageEnglish (US)
Pages (from-to)261-265
Number of pages5
JournalBiotechnic and Histochemistry
Volume51
Issue number5
DOIs
StatePublished - Jan 1 1976
Externally publishedYes

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

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