For application of the Bodian method to frozen sections, cut frozen peripheral nerve or muscle at 10μm and mount. Fix for 4 days in 18% parts 80% ethanol, 1 part 10% formalin, and 1 part glacial acetic acid. Fix central nervous tissue in the same mixture prior to freezing and sectioning, and after mounting postfix for 4 days. Impregnate by the Bodian procedure. The results equal Bodian stains of paraffin sections. The technique is simple and reliable. The use of 10μm frozen sections produces little artifact and allows alternate serial sections to be stained with other techniques.
ASJC Scopus subject areas
- Medical Laboratory Technology