A bodian method for mounted frozen sections

Barbara Evelyn Herr, Paul Coleman, Robert C. Griggs

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

For application of the Bodian method to frozen sections, cut frozen peripheral nerve or muscle at 10μm and mount. Fix for 4 days in 18% parts 80% ethanol, 1 part 10% formalin, and 1 part glacial acetic acid. Fix central nervous tissue in the same mixture prior to freezing and sectioning, and after mounting postfix for 4 days. Impregnate by the Bodian procedure. The results equal Bodian stains of paraffin sections. The technique is simple and reliable. The use of 10μm frozen sections produces little artifact and allows alternate serial sections to be stained with other techniques.

Original languageEnglish (US)
Pages (from-to)261-265
Number of pages5
JournalBiotechnic and Histochemistry
Volume51
Issue number5
DOIs
StatePublished - Jan 1 1976
Externally publishedYes

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Frozen Sections
Nerve Tissue
Peripheral Nerves
Acetic Acid
Paraffin
Artifacts
Formaldehyde
Freezing
Ethanol
Coloring Agents
Muscles

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

Cite this

A bodian method for mounted frozen sections. / Herr, Barbara Evelyn; Coleman, Paul; Griggs, Robert C.

In: Biotechnic and Histochemistry, Vol. 51, No. 5, 01.01.1976, p. 261-265.

Research output: Contribution to journalArticle

Herr, Barbara Evelyn ; Coleman, Paul ; Griggs, Robert C. / A bodian method for mounted frozen sections. In: Biotechnic and Histochemistry. 1976 ; Vol. 51, No. 5. pp. 261-265.
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