A biomedically enriched collection of 7000 human ORF clones

Andreas Rolfs, Yanhui Hu, Lars Ebert, Dietmar Hoffmann, Dongmei Zuo, Niro Ramachandran, Jacob Raphael, Fontina Kelley, Seamus McCarron, Daniel A. Jepson, Binghua Shen, Munira M A Baqui, Joseph Pearlberg, Elena Taycher, Craig DeLoughery, Andreas Hoerlein, Bernhard Korn, Joshua LaBaer

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

We report the production and availability over 7000 fully sequence verified plasmid ORF clones representing over 3400 unique human genes. These ORF clones were derived using the human MGC collection as template and were produced in two formats: with and without stop codons. Thus, this collection supports the production of either native protein or proteins with fusion tags added to either or both ends. The template clones used to generate this collection were enriched in three ways. First, gene radundancy was removed. Second, clones were selected to represent the best available GenBank reference sequence. Finally, a literature-based software tool was used to evaluate the list of target genes to ensure that it broadly reflected biomedical research interest. The target gene list was compared with 4000 human diseases and over 8500 biological and chemical MeSH classes in ∼15 Million publications recorded in PubMed at the time of analysis. The outcome of this analysis revealed that relative to the genome and the MGC collection, this collection is enriched for the presence of genes with published associations with a wide range of diseases and biomedical terms without displaying a particular bias towards any single disease or concept. Thus, this collection is likely to be a powerful resource for researchers who wish to study protein function in a set of genes with documented biomedical significance. Copyright:

Original languageEnglish (US)
Article numbere1528
JournalPLoS One
Volume3
Issue number1
DOIs
Publication statusPublished - Jan 30 2008
Externally publishedYes

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Rolfs, A., Hu, Y., Ebert, L., Hoffmann, D., Zuo, D., Ramachandran, N., ... LaBaer, J. (2008). A biomedically enriched collection of 7000 human ORF clones. PLoS One, 3(1), [e1528]. https://doi.org/10.1371/journal.pone.0001528