A bioconjugate of Lys and Arg mimics biological activity of the Arg - Gly - Asp peptide sequence

Gholamreza Ehteshami, Daniel C. Brune, John C. Lopez, Stephen Massia

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A peptidomimetic, 2-amino-6-[(2-amino-5{guanidino}pentanoyl) amino] hexanoic acid, was synthesized using Lys and Arg to produce a compound that mimics the biological activity of a cell adhesive Arg-Gly-Asp (RGD) peptide, GRGDSP. When immobilized on solid substrates, the peptidomimetic promoted cell adhesion similar to substrates with immobilized GRGDSP. Ligand competition studies demonstrated that cell interactions with the peptidomimetic were integrin-mediated. The peptidomimetic was very stable to proteolytic degradation in comparison to proteolytically unstable peptides. Both GRGDSP and peptidomimetic were stabilized when immobilized on solid substrates. This peptidomimetic has the broad therapeutic utility of the RGD peptides with higher stability and potentially enhanced therapeutic efficacy.

Original languageEnglish (US)
Pages (from-to)85-91
Number of pages7
JournalActa Biomaterialia
Volume1
Issue number1
DOIs
StatePublished - Jan 2005

Fingerprint

Peptidomimetics
Bioactivity
glycyl-arginyl-glycyl-aspartyl-seryl-proline
Peptides
Substrates
Cell adhesion
Amino acids
Adhesives
Ligands
Cells
Degradation
Cell Adhesion
Integrins
Cell Communication
Therapeutics

Keywords

  • Biomaterial coatings
  • Cell adhesion
  • Integrin antagonist
  • RGD peptide mimetic

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

A bioconjugate of Lys and Arg mimics biological activity of the Arg - Gly - Asp peptide sequence. / Ehteshami, Gholamreza; Brune, Daniel C.; Lopez, John C.; Massia, Stephen.

In: Acta Biomaterialia, Vol. 1, No. 1, 01.2005, p. 85-91.

Research output: Contribution to journalArticle

Ehteshami, Gholamreza ; Brune, Daniel C. ; Lopez, John C. ; Massia, Stephen. / A bioconjugate of Lys and Arg mimics biological activity of the Arg - Gly - Asp peptide sequence. In: Acta Biomaterialia. 2005 ; Vol. 1, No. 1. pp. 85-91.
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