@article{39d57e574d7a41098763665a5c4ca2cd,
title = "A BioBrick compatible strategy for genetic modification of plants",
abstract = "Background: Plant biotechnology can be leveraged to produce food, fuel, medicine, and materials. Standardized methods advocated by the synthetic biology community can accelerate the plant design cycle, ultimately making plant engineering more widely accessible to bioengineers who can contribute diverse creative input to the design process.Results: This paper presents work done largely by undergraduate students participating in the 2010 International Genetically Engineered Machines (iGEM) competition. Described here is a framework for engineering the model plant Arabidopsis thaliana with standardized, BioBrick compatible vectors and parts available through the Registry of Standard Biological Parts (http://www.partsregistry.org). This system was used to engineer a proof-of-concept plant that exogenously expresses the taste-inverting protein miraculin.Conclusions: Our work is intended to encourage future iGEM teams and other synthetic biologists to use plants as a genetic chassis. Our workflow simplifies the use of standardized parts in plant systems, allowing the construction and expression of heterologous genes in plants within the timeframe allotted for typical iGEM projects.",
keywords = "Arabidopsis, IGEM, Plant biotechnology, Synthetic biology",
author = "Boyle, {Patrick M.} and Burrill, {Devin R.} and Inniss, {Mara C.} and Agapakis, {Christina M.} and Aaron Deardon and DeWerd, {Jonathan G.} and Gedeon, {Michael A.} and Quinn, {Jacqueline Y.} and Paull, {Morgan L.} and Raman, {Anugraha M.} and Theilmann, {Mark R.} and Lu Wang and Winn, {Julia C.} and Oliver Medvedik and Kurt Schellenberg and Karmella Haynes and Alain Viel and Brenner, {Tamara J.} and Church, {George M.} and Shah, {Jagesh V.} and Silver, {Pamela A.}",
note = "Funding Information: We would like to thank the people at iGEM for supporting this project and allowing us the opportunity to present it at the 2010 iGEM competition. Further thanks are owed to Sarah Mathews of the Harvard Arnold Arboretum for providing advice, access to her lab, and reagents. PMB was supported by the Harvard University Center for the Environment Graduate Consortium. PMB and DRB were supported by the National Science Foundation (NSF) Synthetic Biology Engineering Research Center (SynBERC). MCI was supported by the Natural Science and Engineering Research Council of Canada. CMA was supported by a NSF Graduate Research Fellowship. AD, JGdW, MAG, JYQ, MLP, AMR, MRT, LW, JCW, and OM were generously supported by the Wyss Institute for Biologically Inspired Engineering, Harvard{\textquoteright}s Office of the Provost, and a grant from the Howard Hughes Medical Institute Undergraduate Education Program awarded to Robert A. Lue, Department of Molecular and Cellular Biology, Harvard University. KS was supported by the Arnold Arboretum.",
year = "2012",
month = jun,
day = "20",
doi = "10.1186/1754-1611-6-8",
language = "English (US)",
volume = "6",
journal = "Journal of Biological Engineering",
issn = "1754-1611",
publisher = "BioMed Central",
}