9-Aminoacridine mutagenesis of bacteriophage T4 intracellular DNA

Sidney Altman, V. Warner

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Most of the intracellular T4 DNA made in the presence of 9-aminoacridine is of lower molecular weight than mature T4 DNA and does not get packaged into phage particles. Using a T4 DNA transformation assay, we have examined this intracellular T4 DNA for its content of 9-aminoacridine-induced revertants of certain rII gene frameshift mutations. The proportion of acridine-induced revertants in the intracellular DNA population is close to that found in the phage progeny made in the presence of 9-aminoacridine. Thus, the generation of low molecular weight T4 DNA in the presence of 9-aminoacridine is not, in itself, also a mutagenic process.

Original languageEnglish (US)
Pages (from-to)333-343
Number of pages11
JournalMGG Molecular & General Genetics
Volume138
Issue number4
DOIs
StatePublished - Dec 1975
Externally publishedYes

Fingerprint

Aminacrine
Bacteriophage T4
Mutagenesis
DNA
Bacteriophages
Molecular Weight
Acridines
Frameshift Mutation
Population
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

9-Aminoacridine mutagenesis of bacteriophage T4 intracellular DNA. / Altman, Sidney; Warner, V.

In: MGG Molecular & General Genetics, Vol. 138, No. 4, 12.1975, p. 333-343.

Research output: Contribution to journalArticle

@article{2d29f6a0fe744b9cb1644f36f4abc053,
title = "9-Aminoacridine mutagenesis of bacteriophage T4 intracellular DNA",
abstract = "Most of the intracellular T4 DNA made in the presence of 9-aminoacridine is of lower molecular weight than mature T4 DNA and does not get packaged into phage particles. Using a T4 DNA transformation assay, we have examined this intracellular T4 DNA for its content of 9-aminoacridine-induced revertants of certain rII gene frameshift mutations. The proportion of acridine-induced revertants in the intracellular DNA population is close to that found in the phage progeny made in the presence of 9-aminoacridine. Thus, the generation of low molecular weight T4 DNA in the presence of 9-aminoacridine is not, in itself, also a mutagenic process.",
author = "Sidney Altman and V. Warner",
year = "1975",
month = "12",
doi = "10.1007/BF00264803",
language = "English (US)",
volume = "138",
pages = "333--343",
journal = "Molecular Genetics and Genomics",
issn = "1617-4615",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - 9-Aminoacridine mutagenesis of bacteriophage T4 intracellular DNA

AU - Altman, Sidney

AU - Warner, V.

PY - 1975/12

Y1 - 1975/12

N2 - Most of the intracellular T4 DNA made in the presence of 9-aminoacridine is of lower molecular weight than mature T4 DNA and does not get packaged into phage particles. Using a T4 DNA transformation assay, we have examined this intracellular T4 DNA for its content of 9-aminoacridine-induced revertants of certain rII gene frameshift mutations. The proportion of acridine-induced revertants in the intracellular DNA population is close to that found in the phage progeny made in the presence of 9-aminoacridine. Thus, the generation of low molecular weight T4 DNA in the presence of 9-aminoacridine is not, in itself, also a mutagenic process.

AB - Most of the intracellular T4 DNA made in the presence of 9-aminoacridine is of lower molecular weight than mature T4 DNA and does not get packaged into phage particles. Using a T4 DNA transformation assay, we have examined this intracellular T4 DNA for its content of 9-aminoacridine-induced revertants of certain rII gene frameshift mutations. The proportion of acridine-induced revertants in the intracellular DNA population is close to that found in the phage progeny made in the presence of 9-aminoacridine. Thus, the generation of low molecular weight T4 DNA in the presence of 9-aminoacridine is not, in itself, also a mutagenic process.

UR - http://www.scopus.com/inward/record.url?scp=0016689545&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016689545&partnerID=8YFLogxK

U2 - 10.1007/BF00264803

DO - 10.1007/BF00264803

M3 - Article

C2 - 1152842

AN - SCOPUS:0016689545

VL - 138

SP - 333

EP - 343

JO - Molecular Genetics and Genomics

JF - Molecular Genetics and Genomics

SN - 1617-4615

IS - 4

ER -