3D printed nervous system on a chip

Blake N. Johnson, Karen Z. Lancaster, Ian Hogue, Fanben Meng, Yong Lin Kong, Lynn W. Enquist, Michael C. McAlpine

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Bioinspired organ-level in vitro platforms are emerging as effective technologies for fundamental research, drug discovery, and personalized healthcare. In particular, models for nervous system research are especially important, due to the complexity of neurological phenomena and challenges associated with developing targeted treatment of neurological disorders. Here we introduce an additive manufacturing-based approach in the form of a bioinspired, customizable 3D printed nervous system on a chip (3DNSC) for the study of viral infection in the nervous system. Micro-extrusion 3D printing strategies enabled the assembly of biomimetic scaffold components (microchannels and compartmented chambers) for the alignment of axonal networks and spatial organization of cellular components. Physiologically relevant studies of nervous system infection using the multiscale biomimetic device demonstrated the functionality of the in vitro platform. We found that Schwann cells participate in axon-to-cell viral spread but appear refractory to infection, exhibiting a multiplicity of infection (MOI) of 1.4 genomes per cell. These results suggest that 3D printing is a valuable approach for the prototyping of a customized model nervous system on a chip technology.

Original languageEnglish (US)
Pages (from-to)1393-1400
Number of pages8
JournalLab on a Chip
Volume16
Issue number8
DOIs
StatePublished - Apr 21 2016
Externally publishedYes

Fingerprint

Neurology
Nervous System
Biomimetics
Printing
Biomimetic Materials
3D printers
Infection
Technology
Schwann Cells
Virus Diseases
Drug Discovery
Scaffolds (biology)
Microchannels
Nervous System Diseases
Research
Refractory materials
Extrusion
Axons
Genes
Cells

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Johnson, B. N., Lancaster, K. Z., Hogue, I., Meng, F., Kong, Y. L., Enquist, L. W., & McAlpine, M. C. (2016). 3D printed nervous system on a chip. Lab on a Chip, 16(8), 1393-1400. https://doi.org/10.1039/c5lc01270h

3D printed nervous system on a chip. / Johnson, Blake N.; Lancaster, Karen Z.; Hogue, Ian; Meng, Fanben; Kong, Yong Lin; Enquist, Lynn W.; McAlpine, Michael C.

In: Lab on a Chip, Vol. 16, No. 8, 21.04.2016, p. 1393-1400.

Research output: Contribution to journalArticle

Johnson, BN, Lancaster, KZ, Hogue, I, Meng, F, Kong, YL, Enquist, LW & McAlpine, MC 2016, '3D printed nervous system on a chip', Lab on a Chip, vol. 16, no. 8, pp. 1393-1400. https://doi.org/10.1039/c5lc01270h
Johnson BN, Lancaster KZ, Hogue I, Meng F, Kong YL, Enquist LW et al. 3D printed nervous system on a chip. Lab on a Chip. 2016 Apr 21;16(8):1393-1400. https://doi.org/10.1039/c5lc01270h
Johnson, Blake N. ; Lancaster, Karen Z. ; Hogue, Ian ; Meng, Fanben ; Kong, Yong Lin ; Enquist, Lynn W. ; McAlpine, Michael C. / 3D printed nervous system on a chip. In: Lab on a Chip. 2016 ; Vol. 16, No. 8. pp. 1393-1400.
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