TY - JOUR
T1 - 3D inverted opal hydrogel scaffolds with oxygen sensing capability
AU - Liu, Yuanfang
AU - Wang, Shaopeng
N1 - Funding Information:
This research was supported by DARPA (contract no. W81XWH-04-C-0139) and by an OARS award (AR 03(2)-068) from Oklahoma Center for the Advancement of Science and Technology. Stimulating discussions and helpful advices from Professor Nicholas A. Kotov at University of Michigan are acknowledged.
PY - 2007/7/1
Y1 - 2007/7/1
N2 - The measurement of local oxygen level in 3D cell culture is desired but remains as a challenge problem. We developed a 3D cell scaffold with luminescence-based oxygen sensing capability that opens the possibility of 3D mapping of oxygen level during cell growth. Hydrogel inverted opal scaffold was prepared by photo-polymerization of poly(2-hydroxyethyl methacrylate (pHEMA) and poly(methacryloyloxy)ethyl-trimethylammonium chloride (pMEATAC) monomer using close-packed bead assembly as template. Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium chloride (Ru(dpp)3), was coated on the pHEMA-pMEATAC 3D scaffolds by layer-by-layer (LBL) assembly. pHEMA-pMEATAC copolymer was coated on top of the Ru(dpp)3 layer as a protection layer. The fluorescence emission of Ru(dpp)3 can be dynamically quenched by oxygen. By measuring the emission intensity of the scaffold, the local oxygen level can be monitored. The hydrogel scaffolds are transparent, and thus 3D fluorescence intensity can be mapped by confocal microscopy. Human bone marrow stromal cells HS-5 were successfully cultured on the oxygen sensing scaffold, and the observed Ru(dpp)3 emission intensity from the scaffold was stronger in cell rich area, which indicates a lower oxygen level due to the consumption of the cells.
AB - The measurement of local oxygen level in 3D cell culture is desired but remains as a challenge problem. We developed a 3D cell scaffold with luminescence-based oxygen sensing capability that opens the possibility of 3D mapping of oxygen level during cell growth. Hydrogel inverted opal scaffold was prepared by photo-polymerization of poly(2-hydroxyethyl methacrylate (pHEMA) and poly(methacryloyloxy)ethyl-trimethylammonium chloride (pMEATAC) monomer using close-packed bead assembly as template. Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium chloride (Ru(dpp)3), was coated on the pHEMA-pMEATAC 3D scaffolds by layer-by-layer (LBL) assembly. pHEMA-pMEATAC copolymer was coated on top of the Ru(dpp)3 layer as a protection layer. The fluorescence emission of Ru(dpp)3 can be dynamically quenched by oxygen. By measuring the emission intensity of the scaffold, the local oxygen level can be monitored. The hydrogel scaffolds are transparent, and thus 3D fluorescence intensity can be mapped by confocal microscopy. Human bone marrow stromal cells HS-5 were successfully cultured on the oxygen sensing scaffold, and the observed Ru(dpp)3 emission intensity from the scaffold was stronger in cell rich area, which indicates a lower oxygen level due to the consumption of the cells.
KW - Fluorescence quenching
KW - Inverted opal hydrogel scaffold
KW - Oxygen sensing
KW - Ruthenium dye
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U2 - 10.1016/j.colsurfb.2006.08.014
DO - 10.1016/j.colsurfb.2006.08.014
M3 - Article
C2 - 17005382
AN - SCOPUS:34249053219
SN - 0927-7765
VL - 58
SP - 8
EP - 13
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 1
ER -