TY - JOUR
T1 - 2,4-DNT removal in intimately coupled photobiocatalysis
T2 - The roles of adsorption, photolysis, photocatalysis, and biotransformation
AU - Wen, Donghui
AU - Li, Guozheng
AU - Xing, Rui
AU - Park, Seongjun
AU - Rittmann, Bruce
N1 - Funding Information:
Acknowledgements This study was supported by the US National Science Foundation (Grant No. 0651794) and China Scholarship Council. We sincerely thank Prof. Yongming Zhang at Shanghai Normal University, China, for kindly providing the ceramic carriers and Samsung Engineering Co. Ltd, Korea, for kindly providing the sponge carriers. We also thank Bradley Lusk, who helped in DNA sequencing analysis, and Dr. Rosa Krajmalnik-Brown, who provided guidance and facilities for phylogenetic analysis.
PY - 2012/7
Y1 - 2012/7
N2 - The removal of 2,4-dinitrotoluene (2,4-DNT) by simultaneous UV-photo(cata)lysis and biodegradation was explored using intimately coupled photolysis/photocatalysis and biodegradation (ICPB) with two novel porous carriers. First, a porous ceramic carrier was used to attach the photocatalyst (TiO 2) on its exterior and accumulate biomass in its interior. UV irradiation alone decomposed 71% of the 2,4-DNT in 60 h, and TiO 2 catalyst improved the photolysis to 77%. Second, a macroporous sponge carrier was used to strongly adsorb 2,4-DNT and protect microorganisms from 2,4-DNT inhibition and UV irradiation. The main photolytic reactions were reduction of the nitryl to amino and hydrolysis of the amino to release NH 4 +. The main biodegradation reactions were oxidative release of NO 3 - and accelerated reductive release of NH 4 +. ICPB more thoroughly released inorganic N, with nearly equal amounts being oxidized to nitrate and reduced to ammonium. The genera Burkholderia and Bacillus were found inside the sponge carriers, and they are associated with biodegradation of DNT and its photolysis intermediates. Therefore, using an adsorbent and macroporous biofilm carrier enabled the effective removal of 2,4-DNT by ICPB.
AB - The removal of 2,4-dinitrotoluene (2,4-DNT) by simultaneous UV-photo(cata)lysis and biodegradation was explored using intimately coupled photolysis/photocatalysis and biodegradation (ICPB) with two novel porous carriers. First, a porous ceramic carrier was used to attach the photocatalyst (TiO 2) on its exterior and accumulate biomass in its interior. UV irradiation alone decomposed 71% of the 2,4-DNT in 60 h, and TiO 2 catalyst improved the photolysis to 77%. Second, a macroporous sponge carrier was used to strongly adsorb 2,4-DNT and protect microorganisms from 2,4-DNT inhibition and UV irradiation. The main photolytic reactions were reduction of the nitryl to amino and hydrolysis of the amino to release NH 4 +. The main biodegradation reactions were oxidative release of NO 3 - and accelerated reductive release of NH 4 +. ICPB more thoroughly released inorganic N, with nearly equal amounts being oxidized to nitrate and reduced to ammonium. The genera Burkholderia and Bacillus were found inside the sponge carriers, and they are associated with biodegradation of DNT and its photolysis intermediates. Therefore, using an adsorbent and macroporous biofilm carrier enabled the effective removal of 2,4-DNT by ICPB.
KW - 2,4-DNT
KW - Adsorption
KW - Biodegradation
KW - Intimate coupling
KW - Photolysis
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U2 - 10.1007/s00253-011-3692-6
DO - 10.1007/s00253-011-3692-6
M3 - Article
C2 - 22101782
AN - SCOPUS:84862022514
SN - 0175-7598
VL - 95
SP - 263
EP - 272
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 1
ER -