15-LOX-1 inhibits p21 (Cip/WAF 1) expression by enhancing MEK-ERK1/2 signaling in colon carcinoma cells

Masahiro Yoshinaga; F. Gregory Buchanan; Raymond N. DuBois

doi:10.1016/j.prostaglandins.2004.01.001

15-LOX-1 inhibits p21 (Cip/WAF 1) expression by enhancing MEK-ERK1/2 signaling in colon carcinoma cells

Masahiro Yoshinaga, F. Gregory Buchanan, Raymond N. DuBois

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Currently, some controversy exists regarding the precise role of 15-lipoxygenase-1 (15-LOX-1) in colorectal carcinogenesis and other aspects of cancer biology. The aim of this study was to evaluate the effect of 15-LOX-1 on p21 (Cip/WAF 1) expression and growth regulation in human colon carcinoma cells. The effect of 13-S-hydroxyoctadecadienoic acid (HODE), a product of 15-LOX-1, on p21 (Cip/WAF 1) expression was evaluated in Caco-2 cells treated with sodium butyrate (NaBT) and/or nordihydroguaiarectic acid (NDGA), a LOX inhibitor. The effect of transfecting HCT-116 cells with 15-LOX-1 was also examined. NaBT-induced p21 (Cip/WAF 1) expression was enhanced by treatment with NDGA and 13-S-HODE reversed NaBT-induced p21 (Cip/WAF 1) expression in Caco-2 cells. Overexpression of 15-LOX-1 induced extracellular signal-related kinase (ERK) 1/ 2 phosphorylation, decreased p21 (Cip/WAF 1) expression, and increased HCT-116 cell growth. Treatment with NDGA decreased ERK 1/2 phosphorylation, and increased p21 (Cip/WAF 1) expression in 15-LOX-1 overexpressing HCT-116 cells. Our experimental results support the hypothesis that 15-LOX-1 may have "pro-neoplastic" effects during the development of colorectal cancer.

Original language	English (US)
Pages (from-to)	111-122
Number of pages	12
Journal	Prostaglandins and Other Lipid Mediators
Volume	73
Issue number	1-2
DOIs	https://doi.org/10.1016/j.prostaglandins.2004.01.001
State	Published - Jan 2004
Externally published	Yes

Keywords

13-S-HODE
13-S-hydroxyoctadecadienoic acid
15-LOX-1
15-lipoxygenase-1
ERK
Extracellular signal-related kinase
NDGA
NaBT
Nordihydroguaiarectic acid
PPAR gamma
Peroxisome proliferator-activated receptor gamma
Sodium butyrate
p21 (Cip/WAF 1)

ASJC Scopus subject areas

Biochemistry
Physiology
Pharmacology
Cell Biology

Access to Document

10.1016/j.prostaglandins.2004.01.001

Cite this

@article{2ae0609165bc4837b46b6b5b03d3911a,

title = "15-LOX-1 inhibits p21 (Cip/WAF 1) expression by enhancing MEK-ERK1/2 signaling in colon carcinoma cells",

abstract = "Currently, some controversy exists regarding the precise role of 15-lipoxygenase-1 (15-LOX-1) in colorectal carcinogenesis and other aspects of cancer biology. The aim of this study was to evaluate the effect of 15-LOX-1 on p21 (Cip/WAF 1) expression and growth regulation in human colon carcinoma cells. The effect of 13-S-hydroxyoctadecadienoic acid (HODE), a product of 15-LOX-1, on p21 (Cip/WAF 1) expression was evaluated in Caco-2 cells treated with sodium butyrate (NaBT) and/or nordihydroguaiarectic acid (NDGA), a LOX inhibitor. The effect of transfecting HCT-116 cells with 15-LOX-1 was also examined. NaBT-induced p21 (Cip/WAF 1) expression was enhanced by treatment with NDGA and 13-S-HODE reversed NaBT-induced p21 (Cip/WAF 1) expression in Caco-2 cells. Overexpression of 15-LOX-1 induced extracellular signal-related kinase (ERK) 1/ 2 phosphorylation, decreased p21 (Cip/WAF 1) expression, and increased HCT-116 cell growth. Treatment with NDGA decreased ERK 1/2 phosphorylation, and increased p21 (Cip/WAF 1) expression in 15-LOX-1 overexpressing HCT-116 cells. Our experimental results support the hypothesis that 15-LOX-1 may have {"}pro-neoplastic{"} effects during the development of colorectal cancer.",

keywords = "13-S-HODE, 13-S-hydroxyoctadecadienoic acid, 15-LOX-1, 15-lipoxygenase-1, ERK, Extracellular signal-related kinase, NDGA, NaBT, Nordihydroguaiarectic acid, PPAR gamma, Peroxisome proliferator-activated receptor gamma, Sodium butyrate, p21 (Cip/WAF 1)",

author = "Masahiro Yoshinaga and Buchanan, {F. Gregory} and DuBois, {Raymond N.}",

note = "Funding Information: The authors thank Dr. Brash (Vanderbilt University, Nashville) for kindly providing the cDNA probe of 15-LOX-1 and Dr. Eling (NIEHS, North California) for kindly providing the pcDNA 3.1/15-LOX-1 plasmid. This work was supported in part from the United States Public Health Services Grants R37 DK 47297 Merit Grant (RND), RO1 DK 62112 (RND) P01CA77839 (RND). We thank the T.J. Martell Foundation and the NCCRA (Katie Couric) for their generous support.",

year = "2004",

month = jan,

doi = "10.1016/j.prostaglandins.2004.01.001",

language = "English (US)",

volume = "73",

pages = "111--122",

journal = "Prostaglandins and Other Lipid Mediators",

issn = "1098-8823",

publisher = "Elsevier BV",

number = "1-2",

}

TY - JOUR

T1 - 15-LOX-1 inhibits p21 (Cip/WAF 1) expression by enhancing MEK-ERK1/2 signaling in colon carcinoma cells

AU - Yoshinaga, Masahiro

AU - Buchanan, F. Gregory

AU - DuBois, Raymond N.

N1 - Funding Information: The authors thank Dr. Brash (Vanderbilt University, Nashville) for kindly providing the cDNA probe of 15-LOX-1 and Dr. Eling (NIEHS, North California) for kindly providing the pcDNA 3.1/15-LOX-1 plasmid. This work was supported in part from the United States Public Health Services Grants R37 DK 47297 Merit Grant (RND), RO1 DK 62112 (RND) P01CA77839 (RND). We thank the T.J. Martell Foundation and the NCCRA (Katie Couric) for their generous support.

PY - 2004/1

Y1 - 2004/1

N2 - Currently, some controversy exists regarding the precise role of 15-lipoxygenase-1 (15-LOX-1) in colorectal carcinogenesis and other aspects of cancer biology. The aim of this study was to evaluate the effect of 15-LOX-1 on p21 (Cip/WAF 1) expression and growth regulation in human colon carcinoma cells. The effect of 13-S-hydroxyoctadecadienoic acid (HODE), a product of 15-LOX-1, on p21 (Cip/WAF 1) expression was evaluated in Caco-2 cells treated with sodium butyrate (NaBT) and/or nordihydroguaiarectic acid (NDGA), a LOX inhibitor. The effect of transfecting HCT-116 cells with 15-LOX-1 was also examined. NaBT-induced p21 (Cip/WAF 1) expression was enhanced by treatment with NDGA and 13-S-HODE reversed NaBT-induced p21 (Cip/WAF 1) expression in Caco-2 cells. Overexpression of 15-LOX-1 induced extracellular signal-related kinase (ERK) 1/ 2 phosphorylation, decreased p21 (Cip/WAF 1) expression, and increased HCT-116 cell growth. Treatment with NDGA decreased ERK 1/2 phosphorylation, and increased p21 (Cip/WAF 1) expression in 15-LOX-1 overexpressing HCT-116 cells. Our experimental results support the hypothesis that 15-LOX-1 may have "pro-neoplastic" effects during the development of colorectal cancer.

AB - Currently, some controversy exists regarding the precise role of 15-lipoxygenase-1 (15-LOX-1) in colorectal carcinogenesis and other aspects of cancer biology. The aim of this study was to evaluate the effect of 15-LOX-1 on p21 (Cip/WAF 1) expression and growth regulation in human colon carcinoma cells. The effect of 13-S-hydroxyoctadecadienoic acid (HODE), a product of 15-LOX-1, on p21 (Cip/WAF 1) expression was evaluated in Caco-2 cells treated with sodium butyrate (NaBT) and/or nordihydroguaiarectic acid (NDGA), a LOX inhibitor. The effect of transfecting HCT-116 cells with 15-LOX-1 was also examined. NaBT-induced p21 (Cip/WAF 1) expression was enhanced by treatment with NDGA and 13-S-HODE reversed NaBT-induced p21 (Cip/WAF 1) expression in Caco-2 cells. Overexpression of 15-LOX-1 induced extracellular signal-related kinase (ERK) 1/ 2 phosphorylation, decreased p21 (Cip/WAF 1) expression, and increased HCT-116 cell growth. Treatment with NDGA decreased ERK 1/2 phosphorylation, and increased p21 (Cip/WAF 1) expression in 15-LOX-1 overexpressing HCT-116 cells. Our experimental results support the hypothesis that 15-LOX-1 may have "pro-neoplastic" effects during the development of colorectal cancer.

KW - 13-S-HODE

KW - 13-S-hydroxyoctadecadienoic acid

KW - 15-LOX-1

KW - 15-lipoxygenase-1

KW - ERK

KW - Extracellular signal-related kinase

KW - NDGA

KW - NaBT

KW - Nordihydroguaiarectic acid

KW - PPAR gamma

KW - Peroxisome proliferator-activated receptor gamma

KW - Sodium butyrate

KW - p21 (Cip/WAF 1)

UR - http://www.scopus.com/inward/record.url?scp=1842610142&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842610142&partnerID=8YFLogxK

U2 - 10.1016/j.prostaglandins.2004.01.001

DO - 10.1016/j.prostaglandins.2004.01.001

M3 - Article

C2 - 15165036

AN - SCOPUS:1842610142

SN - 1098-8823

VL - 73

SP - 111

EP - 122

JO - Prostaglandins and Other Lipid Mediators

JF - Prostaglandins and Other Lipid Mediators

IS - 1-2

ER -

15-LOX-1 inhibits p21 (Cip/WAF 1) expression by enhancing MEK-ERK1/2 signaling in colon carcinoma cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this