γ-Tubulin is a component of the Spitzenkorper and centrosomes in hyphal-tip cells of Allomyces macrogynus

D. P. McDaniel, Robert Roberson

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

A monoclonal antibody was used to localize γ-tubulin in hyphal tip cells of the chytridiomycete fungus Allomyces macrogynus, and its distribution determined with standard epifluorescence and laser scanning confocal microscopy. The results demonstrate that γ-tubulin is a component of the Spitzenkorper and centrosomes. Immunoblot analysis of total soluble protein extracts separated by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis identified a single 56 kDa γ-tubulin-related polypeptide. Localization of γ-tubulin to the Spitzenkorper of A. macrogynus provides evidence that the Spitzenkorper in this fungus functions as a microtubule-organizing center.

Original languageEnglish (US)
Pages (from-to)118-123
Number of pages6
JournalProtoplasma
Volume203
Issue number1-2
DOIs
StatePublished - Jan 1 1998

Keywords

  • Immunofluorescence
  • Microtubule-organizing center
  • Spitzenkorper
  • γ-Tubulin

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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