A monoclonal antibody was used to localize γ-tubulin in hyphal tip cells of the chytridiomycete fungus Allomyces macrogynus, and its distribution determined with standard epifluorescence and laser scanning confocal microscopy. The results demonstrate that γ-tubulin is a component of the Spitzenkorper and centrosomes. Immunoblot analysis of total soluble protein extracts separated by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis identified a single 56 kDa γ-tubulin-related polypeptide. Localization of γ-tubulin to the Spitzenkorper of A. macrogynus provides evidence that the Spitzenkorper in this fungus functions as a microtubule-organizing center.
- Microtubule-organizing center
ASJC Scopus subject areas
- Plant Science
- Cell Biology