Abstract
A monoclonal antibody was used to localize γ-tubulin in hyphal tip cells of the chytridiomycete fungus Allomyces macrogynus, and its distribution determined with standard epifluorescence and laser scanning confocal microscopy. The results demonstrate that γ-tubulin is a component of the Spitzenkorper and centrosomes. Immunoblot analysis of total soluble protein extracts separated by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis identified a single 56 kDa γ-tubulin-related polypeptide. Localization of γ-tubulin to the Spitzenkorper of A. macrogynus provides evidence that the Spitzenkorper in this fungus functions as a microtubule-organizing center.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 118-123 |
| Number of pages | 6 |
| Journal | Protoplasma |
| Volume | 203 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Jan 1 1998 |
Keywords
- Immunofluorescence
- Microtubule-organizing center
- Spitzenkorper
- γ-Tubulin
ASJC Scopus subject areas
- Plant Science
- Cell Biology