α/β Hydrolase2, a predicated gene adjacent to Mad in Drosophila melanogaster, belongs to a new global multigene family and is associated with obesity

Robert G. Wisotzkey, Aaron N. Johnson, Norma T. Takaesu, Stuart Newfeld

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The experimental validation of genes predicted from genomic sequence and the identification of functions for these genes is an increasingly important task. We report a multidisciplinary analysis of CG3488, a predicted gene adjacent to Mothers against dpp in Drosophila melanogaster. We cloned and sequenced a cDNA corresponding to CG3488 and we show that it is expressed in embryos. A computational analysis shows that CG3488 contains a number of conserved domains present in enzymes capable of lipid hydrolysis. A phylogenetic analysis shows that CG3488 is the homolog of human α/β hydrolase2 and that these genes belong to a novel multigene family with members in animals, plants, fungi, and bacteria. A genetic analysis shows that heterozygosity for a chromosomal deletion that removes CG3488 dominantly enhances the excess lipid phenotype associated with a mutation in adipose, an uncloned obesity gene. Further, overexpression of a CG3488 transgene rescues this obesity phenotype. Overall, the data suggests that CG3488 functions as a lipase and that analyses of its homologs will provide unique insights into lipid metabolism in many species.

Original languageEnglish (US)
Pages (from-to)351-361
Number of pages11
JournalJournal of Molecular Evolution
Volume56
Issue number3
DOIs
StatePublished - Mar 1 2003

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Keywords

  • CG3488
  • Drosophila
  • Lipase
  • Multigene family
  • Obesity
  • Predicted genes
  • adipose
  • α/β hydrolase2

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

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