The objective of the proposed research is to characterize the comprehensive proteomic response of human cells during spaceflight before and after infection with a microbial pathogen. Specifically, we will quantitatively analyze the global proteins (proteome) expressed in human intestinal cells before and after Salmonella infection during spaceflight cultivation using cells from our STLIMMUNE flight experiment flown in April 2010 aboard STS-131. This landmark experiment represents the first infection of human cell culture in flight and a full utilization of all of the components of this experiment benefit NASAs goals to understand and mitigate infectious disease risk to the crew during spaceflight missions. Knowledge gained from this work is critical for long-term exploration and habitation of space, and holds exciting promise for translation to novel countermeasures for mitigating risk to crew health during spaceflight and for an improved understanding of human health and disease on Earth. Two different human epithelial cell lines from this flight experiment representing model intestinal and lung epithelium will be used in these studies and their comprehensive quantitative proteomic responses to the stress of spaceflight and (for the intestinal cells) microbial pathogen challenge will be independently profiled using iTRAQ (isobaric tags for relative and absolute quantitation). The Gram negative pathogen selected for use in the intestinal infection study, S. typhimurium, has been shown by us to exhibit increased virulence and global alterations in gene expression during culture in spaceflight and spaceflight analogue conditions. All flight study results will be compared to those obtained when the same experiments are performed with identical cells cultured as synchronous ground controls at the Kennedy Space Center in the same hardware used for flight (the Cell Culture Module/CCM).
|Effective start/end date||7/1/10 → 9/30/11|
- NASA: Johnson Space Center: $30,000.00