WET Center Project: Field Study of Legionella in Waters: Characterization of species complex

Project: Research project

Project Details

Description

WET Center Project: Field Study of Legionella in Waters: Characterization of species complex Field Study of Legionella in Waters: Characterization of species complex SCOPE OF WORK Legionella bacteria are ubiquitous in natural and artificial water supplies where protozoan host endoparasitization and biofilm colonization are involved in their life cycle. Humans can become incidental hosts, resulting in legionellosis. Current Legionella knowledge is severely lacking in several key areas, particularly concerning the interactions between the bacteria, the biofilms they live in, and their host organisms. Experiments planned included using laboratory scale model water distribution systems consisting two independent pipe loops constructed of cast iron and PVC with continuously recirculated water at a flow of 1 foot per second. Tap water from the city of Tempe, Arizona, is received in an open tank to allow chlorine evaporation before being transferred by gravity to each of the reservoirs. The two models are used to examine the dissemination of Legionella into water system biofilms. In addition, coupons are added to each reservoir for biofilm formation. After several weeks of biofilm development, L. pneumophila strain Knoxville-1 will be inoculated into the systems. Through the course of several weeks, water and biofilm samples are collected and analyzed for presence of Legionella. While initial experiments with these systems are currently being performed, data suggest that Legionella quickly associate with system biofilms, as none can be detected in the bulk water of either system 6 hours after inoculation. Further research will determine the time that Legionella can be maintained within the systems.
StatusFinished
Effective start/end date5/1/134/30/14

Funding

  • INDUSTRY: Various Consortium Members: $26,146.00

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