Uncovering How Serotonergic Signaling Non-Autonomously Regulates Protein Homeostasis

Uncovering How Serotonergic Signaling Non-Autonomously Regulates Protein Homeostasis Uncovering how serotonergic signaling non-autonomously regulates protein homeostasis The goal of this research proposal is determine how thermosensory neurons can trigger the heat shock response in other cells/tissues. Preliminary results led us to hypothesize that thermosensory-induced release of serotonin activates adaptive cellular stress responses that protect protein homeostasis. We will test this hypothesis in the round nematode C. elegans, a well-established model system where thermosensoryinduced serotonin signaling has been thoroughly investigated. We anticipate that this approach will yield a large number of exciting candidate genes, which when characterized as described, will inform us on the cellular changes that accompany. Services to be performed: 1) Preparation of polyPull vector Punc-119::GFP::pab-1 2) Preparation of 1 MoSCI line with Punc-119::GFP::pab-1 3) 12 PAT-Seq RNA immunoprecipitation reactions. 4) cDNA library preparation for 12 samples using SPIA technology. 5) NextGen sequencing using Illumina platform. 6) Data analysis and validation.