SA 2. Optimize Electrophoresis (EP) and Dielectrophoresis (DEP) for Biofluids Biological particles and molecular species can be influenced and separated by electrokinetic effects. 79, 102, 103 104-106 These methods use both linear electric fields and field gradients demonstrating separation using electrophoresis or dielectrophoresis or a combination of the two. We propose to exploit and explore the full capabilities of electrokinetic separatory effects in these devices where unwanted materials from environmental contaminants, molecular species and biological debris can be sequestered and the desired materials can be localized and concentrated. We will use the well established capabilities (see 35, 36, 85-88) of electrophoresis to separate small molecules, peptides and proteins. We will use two emerging strategies, one where a flow field is used in the presence of electrophoretic forces (addressable electrophoretic capture), and a second, where a series of dielectrophoretic gradient traps of increasing intensity are created along a microdevice channel (gradient dielectrophoresis, GDEP). For SA 2, we will be developing these in parallel and separately.
|Effective start/end date||5/15/09 → 4/30/11|
- HHS: National Institutes of Health (NIH): $198,557.00
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