NOVEL APPROACHES TO STUDYING THE SITU BIOREMEDIATION OF COMPLEX MIXTURES

Project: Research project

Description

The in situ microcosm array (ISMA) technology relies on a self-contained, patent-pending submersible device featuring a large number of microcosms that are arranged in parallel, thereby allowing for the execution of multiple experiments in situ in parallel at the same time in the same place. Prior to deployment and incubation of the device in the target environment of interest, the individual microcosms can be seeded with microorganisms as desired. Following in situ incubation and retrieval of the tool from a groundwater monitoring well, the device can be analyzed by chemical, genomic and proteomic techniques. Discoveries made during performance of the original project plan are now opening new opportunities to increase the reach and value of the ISMA technology and to accelerate the pace of science. We propose to broaden the scope of 1R01ES015445 by (i) adapting the ISMA for use in anaerobic environments via design of an integrated nutrient injection module, (ii) studying the fate of trichloroethene in sediment from Lawrence Livermore National Lab Site 300 with and without bioaugmentation, and (iii) determining the influence of the antimicrobial compound triclocarban on TCE dechlorination in anaerobic groundwater. This project will yield as a final and tangible product a novel functionality of the ISMA in the form of a nutrient injection unit capable of delivering a constant stream of water-soluble carbon and energy sources to sediment microcosms so as to achieve and maintain anaerobic conditions in the ISMA device. The scope of work of the supplement is directly related to Specific Aims 1 4 of the parent grant. The introduction of new hardware components for the ISMA will expand the utility of the device by making it suitable for studying the biotransformation of EPA priority pollutants and chemical mixtures both aerobically and anaerobically side-by-side in the same monitoring well at the same time. This project will have a notable and traceable impact on U.S. employment and the economy by supporting a doctoral student, providing training opportunities for up to 4 interns over the course of 2 years, and by supporting supply chain businesses in the U.S. that specialize in fine chemicals and instrumentation hardware components.
StatusFinished
Effective start/end date5/6/087/31/12

Funding

  • HHS: National Institutes of Health (NIH): $553,457.00

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bioremediation
microcosm
trichloroethylene
hardware
incubation
well
groundwater
proteomics
in situ
nutrient
submersible
biotransformation
dechlorination
monitoring
anoxic conditions
sediment
instrumentation
genomics
student
microorganism