Multi-Specific aptamer-nanoscaffolds to induce aptamer-dependent cellualr cytotoxicity (ApDCC) against breast cancer cells

Project: Research project

Description

The proposed study is based on a multivalent and cooperative interaction that is a fundamental process underlying the activation, expansion and execution of protective immunity against various targets, including cancers. NK cell-dependent killing of cancer cells can be triggered upon engagement of activators on the NK cells with ligands on the tumor cells, including antibody-dependent cellular cytotoxicity (ADCC)1. Thus, it is conceivable that aptamers, known as chemical antibodies2, can be exploited for ADCC as well, by inducing aptamer-dependent cellular cytotoxicity (ApDCC). For this purpose, NK-specific and tumor specific aptamers can be linked by DNA, which is an ideal scaffolding material, due to its feasibility of being self-assembled into highly ordered structures, according to simple Watson-Crick base pairing 3.
StatusFinished
Effective start/end date9/15/091/14/11

Funding

  • DOD-ARMY: Army Medical Research Acquisition Activity (USAMRAA) : $98,578.00

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Cytotoxicity
Cells
Tumors
Antibodies
Chemical activation
Ligands
DNA