Identifying Cellular Toxicity Mechanism of Different Type of Nanoparticles in Water

Project: Research project

Project Details

Description

Identifying Cellular Toxicity Mechanism of Different Type of Nanoparticles in Water Identifying Cellular Toxicity Mechanism of Different Type of Nanoparticles in Water Potential routes of nanoparticles exposure to human include inhalation, dermal contact, and ingestion. Toxicology of inhalation of micron and nanometer sized particles has been extensively studied; however, risks of nanoparticles exposure via ingestion are not well understood. In the human body, ingested food is transported through the esophagus into the stomach, where it is exposed to low pH for about 0.5 to 4 hours. Thereafter food is passed through duodenum into intestine. Small intestinal epithelial cells form a monolayer lining the surface of the small intestinal lumen. The epithelial cells separate the intestinal lumen from the systemic circulation and prevent the uptake of nanoparticles, toxic compounds and invasive pathogens through the GI tract. In this study we used Caco-2 cells as a small intestine epithelial model. The possible toxicity of Titanium (Ti), Nickel (Ni), Aluminum (Al), Iron (Fe), Zirconium (Zr) and Zinc (Zn) nanoparticles exposure via ingestion will be investigated. Effect of acidity of gastric fluid treatment on nanoparticles toxicity will also be studied. Commercially available NiO2, ZrO2, ZnO, TiO2, FeO2 and AlO2 nanoparticles will be applied to Caco-2 cells grown in multiwall cell culture plates. Cell viability will be studied after 24 hours of incubation. The experimental plan will include Titanium, Nickel, Aluminum, Iron, and Zirconium and Zinc based nanoparticles. Prior to toxicity assays, nanoparticles will be soaked in acidified Hanks balanced salt solution to study the affect of acid treatment on the toxicity profile of nanoparticles.
StatusFinished
Effective start/end date11/15/105/15/12

Funding

  • INDUSTRY: Various Consortium Members: $43,000.00

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