Genome sequencing and metabolic analysis is proposed for seven photosynthetic prokaryotes. Organisms are chosen so as to provide significantly wider coverage of genomes of phototrophic taxa than is now available. The broad goals of the project are to use the genomic data that is determined to understand better the origin and evolution of photosynthesis and to explore mechanisms of the anoxygenic to oxygenic transition. The organisms that will be sequenced include species that live at low temperature (psychrophilic), high temperature (thermophilic), high pH (alkaliphilic), in environments subject to periodic drying, and environments high in sulfide. The organisms chosen include two heliobacteria, Heliorestis convoluta and Heliophilum fasciatum (draft sequence); four proteobacteria, Rhodoferax antarcticus, Rhodopila globiformis, Blastochloris viridis (draft sequence), and Thermochromatium tepidum; and one cyanobacterium, Leptolyngbya (a.k.a. Oscillatoria) amphigranulata (draft sequence). The proteobacteria include members from the beta and gamma divisions and one that contains bacteriochlorophyll b as its principal photopigment. Genome sequences of these carefully selected organisms will fill large gaps in the available genomic data for photosynthetic prokaryotes. The organisms will also be analyzed with respect to metabolic capabilities using pathway analysis software tools. Each organism has individual characteristics that justify its inclusion in a genome-sequencing project, including evolutionary relationships, agricultural applications and environmental aspects.
|Effective start/end date||9/1/09 → 8/31/14|
- NSF: Directorate for Biological Sciences (BIO): $867,133.00