Establishment of Conditionally Reprogrammed Breast Cell Lines

Establishment of Conditionally Reprogrammed Breast Cell Lines Establishment of Conditionally Reprogrammed Breast Cell Lines Project Scope/Protocol: A fundamental limitation in breast tumor biology research is the lack of relevant in vitro models for tumor biology research and drug sensitivity testing. Most of the existing cell lines are derived from advanced tumors with markedly abnormal karyotypes and limited relevance to the vast majority of primary breast tumors that are seen in clinic. The goal of this study is to establish well-characterized novel primary cultures of breast tumor epithelial cells for functional analyses and high throughput drug sensitivity testing. Ms. Ting Li (research technician) will work full time at Mayo Clinic (Shea campus) on Mondays and Thursdays (40% effort) performing cell culture to establish and maintain primary cells. She will spend an additional 10% effort on logistical coordination of the samples between the Mayo Hospital, Mayo Shea campus, and the Anderson lab at ASU.