ASU and the NASA Glenn Research Center propose to combine two relatively new techniques in order to change the existing paradigm on how proteins can be separated from a complex biological fluid. In order to explore the early-stage idea of controlling drop splitting via light scattering detection in a single drop, funding from the Early- Concept Grants for Exploratory Research program is well suited because it is a high risk and high payoff project. The paradigm of light scattering visualization as a means of controlling separation in a single drop would represent a fundamental change from current state of the art bioseparations in many ways: (1) there is no confinement of the liquid samples within a capillary, gel plate, or tube; (2) complex biological samples can be used; (3) simultaneous molecular weight and isoelectric point detection of proteins coincident with the separation process; (4) sequences of fractionations can be programmed and accomplished within minutes using the digital (e.g., drop-wise) nature of the liquid handling; and (5) the ability to modify focusing conditions on-thefly to better identify the proteins being detected during separation.
|Effective start/end date||8/15/09 → 4/30/11|
- National Science Foundation (NSF): $49,921.00
Dynamic light scattering