Dynamic Properties that Enhance Enzyme Function

Project: Research project

Description

The long term goals of this research include the development of methodology to permit the folding, flexibility and dynamics of protein structure to be interrogated, and the development and use of fluorescent probes in novel ways to enhance our understanding of protein structure, dynamics and function. During the five years of proposed research, the focus of efforts will be on study of the mechanism of modified DHFRs through steady state, pre-steady state and single molecule kinetic studies. Fluorescence spectroscopy will be used to study the protein conformational changes and their association with the key steps in DHFR catalysis.
StatusFinished
Effective start/end date4/1/103/31/15

Funding

  • HHS-NIH: National Institute of General Medical Sciences (NIGMS): $1,273,285.00

Fingerprint

Enzymes
Proteins
Fluorescence spectroscopy
Fluorescent Dyes
Catalysis
Association reactions
Molecules
Kinetics