The goal of the research proposed in this application is to further characterize the role of an mGluR5-PKC8 signaling pathway in regulating ethanol consumption. This research involves analyzing the effects of local blockade of mGluR5 and/or PKC8 function in specific brain regions on voluntary ethanol self-administration in rats. In addition, the effects of such manipulations on cue-induced reinstatement of ethanol-seeking behavior will also be examined, Finally, biochemical studies examining the role of PKC8 in regulating the cell surface expression of mGluR5 receptors will also be conducted, I am qualified to be the Principal Investigator of this application because I have over 10 years of experience performing intracranial microinjections and determining their effects on ethanol self-administration and reinstatement, and I have 2 years of experience with receptor cell surface expression assays, Thus, I have experience with all of the technical challenges that may be involved with these projects,
|Effective start/end date||11/1/10 → 4/30/15|
- HHS: National Institutes of Health (NIH): $1,337,128.00
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