depth, yet we must consider the impact of their natural environment to understand how folding proceeds naturally in cell, in which folding is directly coupled to protein synthesison ribosome. Although a wealth of biophysical and biochemical data has advanced our general knowledge about the significance of co-translational folding, elucidating the molecular details of the conformational preferences populated by the growing nascent chain (NC) is crucial to better understand co-translational folding. Here we aim to identify the structural and dynamical description of NC through multiscale simulations where every stage of simulations will be verified by experimental studies. With synergistics work of modeling and experiments we will provide insights about: (i) how the folding acquisition of the growing NC differs from the refolding of protein in vitro (ii) How the NC interacts with the ribosome. The results of this study will provide key insights about the co-translational folding and create the basis for the development of new antibiotics. Furthermore, considering the various protein misfolding diseases, an understanding of the folding in the context of translation could provide new directions for the treatments against such diseases.
|Effective start/end date||3/15/10 → 2/28/11|
- National Science Foundation (NSF): $5,831.00