Biocompatible Metal (Core)-Ceramic (Shell) Nanoparticles for siRNA Delivery

Project: Research project

Project Details

Description

NSF Grant CBET 0829128 supports an investigation into biocompatible gold nanorod core-Layered Double Hydroxide (LDH) shell nanoparticles as novel multifunctional nanoscale drug delivery vectors for simultaneous targeted siRNA delivery and hyperthermic ablation. LDH nanoparticles (LDHNs) are a class of inorganic ceramic materials with a general formula M2+ 1-xM3+ x(OH)2.(An-)x/n.mH2O, where M2+ is a divalent cation, M3+ is a trivalent cation, and An- is the interlayer anion of valence n. The unique structure of LDHNs readily allows the intercalation of anionic molecules (e.g. siRNA), into the layers of the nanoparticle via ion exchange. This material is attractive in drug delivery applications due to its biocompatibility and selective degradability induced by acidic environments in late endosomes inside cells. The nanoparticles are designed to possess: 1) gold nanorod core for hyperthermic ablation and optical imaging using near infra-red (NIR) light and 2) intercalated siRNA within the outer LDH shell for overcoming cellular heat-shock response to NIR-based photothermal therapy.

Description

Our research, supported by NSF Grant CBET 0829128 since September 2008, involves an investigation into biocompatible gold nanorod core-Layered Double Hydroxide (LDH) shell nanoparticles as novel multifunctional nanoscale drug delivery vectors for simultaneous targeted siRNA delivery and hyperthermic ablation. LDH nanoparticles (LDHNs) are a class of inorganic ceramic materials with a general formula M2+ 1-xM3+ x(OH)2.(An-)x/n.mH2O, where M2+ is a divalent cation, M3+ is a trivalent cation, and An- is the interlayer anion of valence n. The unique structure of LDHNs readily allows the intercalation of anionic molecules (e.g. siRNA), into the layers of the nanoparticle via ion exchange. This non-polymeric material is attractive in drug delivery applications due to its biocompatibility and selective degradability induced by acidic environments in late endosomes inside cells. Our nanoparticles are characterized by: 1) gold nanorod core for hyperthermic ablation and optical imaging using near infra-red (NIR) light and 2) intercalated siRNA within the outer LDH shell for overcoming cellular heat-shock response to NIR-based photothermal therapy.

Description

Our research, supported by NSF Grant CBET 0829128 since September 2008, involves biocompatible gold nanorod core-Layered Double Hydroxide (LDH) shell nanoparticles as novel multifunctional nanoscale vectors for simultaneous siRNA delivery and hyperthermic ablation of cancer cells. The nanoparticles are designed to have 1) gold nanorod core for hyperthermic ablation and 2) intercalated siRNA within the outer LDH shell for overcoming cellular heat-shock response to NIR-based photothermal therapy. The PI and co-PI currently have two graduate students who work 50% of their research time on the funded NSF project. The student in Dr. Reges group, Mr. Huang-Chiao Huang, investigates gold nanorod-induced hyperthermia in cancer cells, while the graduate student in Dr. Deys group (Mr. Colton Rearick) devotes his time on the generation and characterization of gold-LDH core-shell nanoparticles. Supplemental funding for an additional doctoral student will enable us to enhance the biological portions of the proposed research as described below

Description

The NSF CBET Grantee Conference provides the opportunity for building more effective and collaborative relationships amongst the community of CBET grantees and Program Directors. The conference is designed to enable awardees to share their technical achievements with the NSF Program Directors and also to learn about current (and/or new) NSF/CBET programs, practices, and supplemental funding mechanisms. CBET Program and Cluster-specific sessions will also facilitate discussions amongst these smaller communities to address topics of specific interest. The conference agenda will provide multiple outlets for networking and interaction with program officers and other grantees in efforts to promote discussions and potential collaborations.
StatusFinished
Effective start/end date9/1/088/31/14

Funding

  • National Science Foundation (NSF): $378,729.00

Fingerprint

Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.