An Integrated Metagenomics and Immunoproteomics Study of the Role of Microbiome in Pouchitis Development

Project: Research project

Project Details

Description

An Integrated Metagenomics and Immunoproteomics Study of the Role of Microbiome in Pouchitis Development An Integrated Metagenomics and Immunoproteomics Study of the Role of Microbiome in Pouchitis Development Patient recruitment and sample collection are critical to translational research; however, such efforts receive little support, especially for pilot studies. Our primary goal is to establish an Arizona-based patient recruitment and sample collection infrastructure with a wide network of gastroenterologists in Maricopa County. This infrastructure will help to generate preliminary data for our proposed study, provide the support for future grant applications, and benefit pilot studies on other GI diseases. The complex gut microbiota and their metabolites profoundly influence human health, and dysbiosis of the gut microbiome leads to disease. Although important, it has been challenging to study the role of the microbiome in the development of chronic diseases. We have been studying the role of the microbiome in patients with inflammatory bowel disease including ulcerative colitis and Crohns disease. We propose to use pouchitis, the inflammation of the ileal pouch after a restorative proctocolectomy (RPC) with the ileal pouch anal anastomosis (IPAA) in patients with refractory ulcerative colitis, as a human model that allows the opportunity to further our understanding of the role of the microbiome in the development of inflammation experienced in inflammatory bowel disease. We will take an integrated metagenomics and immunoproteomics approach to compare the gut microbiome and the anti-bacteria immune response in patients who do and do not develop pouchitis. Our ultimate goal is to develop biomarkers that predict pouchitis development so that patients can receive appropriate treatment accordingly. In addition, we want to understand the mechanism and contributing bacteria that lead to its development so that we may develop innovative strategies to prevent its occurrence.
StatusActive
Effective start/end date11/1/1810/31/23

Funding

  • Flinn Foundation: $200,000.00

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