4-D molecular tracking using kilohertz framerate multi-modal microscopy

Project: Research project

Project Details

Description

4-D molecular tracking using kilohertz framerate multi-modal microscopy 4-D molecular tracking using kilohertz framerate multi-modal microscopy Understanding the mechanisms that drive dynamic processes deep within living organisms requires visualizing molecules and ions within rapidly moving sub-cellular structures. One of the most rapid sub-cellular structures is the motile cilium, which is a10 um long whip with a 200 nm diameter that extends from the cell surface. Motile cilia undulate up to 30 times per second to generate hydrodynamic force that propels spinal fluid through the brain, mucus through the respiratory tract, gametes through the reproductive tract, and patterning molecules across the surface of the early embryo (Lindemann and Lesich, 2021). Pathologies that disrupt ciliary beating lead to devastating clinical outcomes that include hydrocephalus, chronic respiratory disease, infertility, and congenital organ malformations (Reiter and Leroux, 2017). Although ciliary beating has been imaged, the dynamic processes that take place within beating cilia have only been investigated in artificially immobilized cilia (Ishikawa and Marshall, 2017). This limitation means that we have an inadequate understanding of a fundamental process that severely reduces the quality of life for millions of people worldwide, including the lethal effects of SARS-Cov2 which decreases cilia function in the respiratory system. The goal of this collaborative Scialog proposal is to develop a multi-modal microscopy technique with sufficient spatial and temporal resolution to track individual particles within freely beating cilia. To achieve our goal, we will blend rapid quantitative phase imaging (Shepherd lab), fluorescence microscopy of individual molecules in beating cilia (Galati lab), and predictive particle tracking within the ciliary waveform (Quinn lab).
StatusFinished
Effective start/end date8/1/217/31/22

Funding

  • Research Corporation for Science Advancement: $55,000.00

Fingerprint

Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.