3-D Organotypic Post-Menopausal Vaginal Epithelial Model for Correlation between Commensal Bacterial Colonization and Mucosal Health

Project: Research project

Project Details

Description

3-D Organotypic Post-Menopausal Vaginal Epithelial Model for Correlation between Commensal Bacterial Colonization and Mucosal Health 3-D Organotypic Post-Menopausal Vaginal Epithelial Model for Correlation between Commensal Bacterial Colonization and Mucosal Health Abstract This project will develop a physiologically relevant 3-D model of post-menopausal vaginal epithelium as a robust human surrogate platforms to assess the impact of colonization by commensal bacteria on mucosal homeostasis to overcome the difficulties and limitations associated with in vivo and in vitro models. This 3-D model will represent an advanced, highly accessible platform with which to meaningfully assess host-microbe interactions. We will use innovative bioengineering technology designed by NASA, the RWV bioreactor, to accomplish our advanced model development. The RWV offers a unique and innovative approach not previously applied toward studying the interactions between commensal microbes and vaginal epithelium, and will provide a novel model system to explore the impact of this process on the health of post-menopausal vaginal mucosa. In addition, commensal bacteria to be used for colonization studies will be cultured under physiological fluid shear forces normally encountered in the vaginal tract to provide a more realistic modeling of the dynamic interactions that occur between microorganisms and the host that may unveil novel insights into the impact of the vaginal microbiome on the dynamic function of this mucosal tissue. Collectively, these approaches will provide a more complete understanding of the dynamic physical and chemical interactions that occur between microorganisms and post-menopausal vaginal epithelium and will lead to improved strategies for ensuring mucosal health.
StatusFinished
Effective start/end date9/1/125/31/14

Funding

  • INDUSTRY: Domestic Company: $131,386.00

Fingerprint

Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.