Research Output 1989 2018

2018

A plant-produced vaccine protects mice against lethal West Nile virus infection without enhancing Zika or dengue virus infectivity

Lai, H., Paul, A. M., Sun, H., He, J., Yang, M., Bai, F. & Chen, Q. Jan 1 2018 (Accepted/In press) In : Vaccine.

Research output: Contribution to journalArticle

Zika virus
West Nile virus
Dengue virus
Dengue Virus
Virus Diseases
1 Citations

Development of antibody therapeutics against flaviviruses

Sun, H., Chen, Q. & Lai, H. Jan 1 2018 In : International Journal of Molecular Sciences. 19, 1, 54

Research output: Contribution to journalReview article

Flavivirus
Monoclonal antibodies
antibodies
Antibodies
Monoclonal Antibodies
2 Citations

Plant-produced Zika virus envelope protein elicits neutralizing immune responses that correlate with protective immunity against Zika virus in mice

Yang, M., Sun, H., Lai, H., Hurtado, J. & Chen, Q. Feb 1 2018 In : Plant Biotechnology Journal. 16, 2, p. 572-580 9 p.

Research output: Contribution to journalArticle

Zika virus
Viral Envelope Proteins
Nicotiana benthamiana
Medicinal Plants
neutralization
2017
5 Citations

Immunization of Zika virus envelope protein domain III induces specific and neutralizing immune responses against Zika virus

Yang, M., Dent, M., Lai, H., Sun, H. & Chen, Q. 2017 (Accepted/In press) In : Vaccine.

Research output: Contribution to journalArticle

Zika virus
Viral Envelope Proteins
neutralization
Immunization
immunization
8 Citations

Virus-like particles that display Zika virus envelope protein domain III induce potent neutralizing immune responses in mice

Yang, M., Lai, H., Sun, H. & Chen, Q. Dec 1 2017 In : Scientific Reports. 7, 1, 7679

Research output: Contribution to journalArticle

Viral Envelope Proteins
Virion
Hepatitis B Core Antigens
Dengue Virus
Vaccines
2016
Agrobacterium
MicroRNAs
Tobacco
Antibodies
Agrobacterium tumefaciens
3 Citations
7 Citations

Plant-produced anti-dengue virus monoclonal antibodies exhibit reduced antibody-dependent enhancement of infection activity

Dent, M., Hurtado, J., Paul, A. M., Sun, H., Lai, H., Yang, M., Esqueda, A., Bai, F., Steinkellner, H. & Chen, Q. Dec 1 2016 In : Journal of General Virology. 97, 12, p. 3280-3290 11 p., 000635

Research output: Contribution to journalArticle

Antibody-Dependent Enhancement
Dengue Virus
Monoclonal Antibodies
Glycosylation
Infection
1 Citations
Human Development
Biological Products
Plant Proteins
Bioterrorism
Drug Approval
7 Citations

Transient protein expression by agroinfiltration in lettuce

Chen, Q., Dent, M., Hurtado, J., Stahnke, J., McNulty, A., Leuzinger, K. & Lai, H. May 1 2016 Methods in Molecular Biology. Humana Press Inc., Vol. 1385, p. 55-67 13 p. (Methods in Molecular Biology; vol. 1385)

Research output: Chapter in Book/Report/Conference proceedingChapter

Lettuce
Recombinant Proteins
Proteins
Agrobacterium tumefaciens
Agriculture
2015
8 Citations

Gene delivery into plant cells for recombinant protein production

Chen, Q. & Lai, H. 2015 In : BioMed Research International. 2015, 932161

Research output: Contribution to journalArticle

Plant Cells
Recombinant Proteins
Genes
Scalability
Transgenes
6 Citations

Plant-made vaccines against West Nile virus are potent, safe, and economically feasible

Chen, Q. May 1 2015 In : Biotechnology Journal. 10, 5, p. 671-680 10 p.

Research output: Contribution to journalArticle

West Nile Virus Vaccines
Vaccines
West Nile virus
Costs and Cost Analysis
Economics
10 Citations

Purification of monoclonal antibody against Ebola GP1 protein expressed in Nicotiana benthamiana

Fulton, A., Lai, H., Chen, Q. & Zhang, C. Apr 10 2015 In : Journal of Chromatography A. 1389, p. 128-132 5 p.

Research output: Contribution to journalArticle

Plant Proteins
Chromatography
Polyelectrolytes
Tobacco
Purification
2014
18 Citations

A plant-produced antigen elicits potent immune responses against west nile virus in mice

He, J., Peng, L., Lai, H., Hurtado, J., Stahnke, J. & Chen, Q. 2014 In : BioMed Research International. 2014, 952865

Research output: Contribution to journalArticle

Plant Antigens
West Nile virus
Viruses
Vaccines
Immunization
26 Citations

Generation and analysis of novel plant-derived antibody-based therapeutic molecules against West Nile virus

He, J., Lai, H., Engle, M., Gorlatov, S., Gruber, C., Steinkellner, H., Diamond, M. S. & Chen, Q. Mar 27 2014 In : PLoS One. 9, 3, e93541

Research output: Contribution to journalArticle

Plantibodies
West Nile virus
Antibody-Dependent Enhancement
Viruses
Monoclonal Antibodies
5 Citations

Plant-derived monoclonal antibodies as human biologics for infectious disease and cancer

Chen, Q. & Lai, H. Oct 29 2014 Plant-derived Pharmaceuticals: Principles and Applications for Developing Countries. CABI International, p. 42-75 34 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Plantibodies
Engine mountings
Biological Products
Communicable Diseases
Monoclonal Antibodies
6 Citations

Plant-made biologics

Chen, Q., Santi, L. & Zhang, C. 2014 In : BioMed Research International. 2014, 418064

Research output: Contribution to journalArticle

Plant Extracts
Biological Products
28 Citations

Structural and functional characterization of an anti-West Nile virus monoclonal antibody and its single-chain variant produced in glycoengineered plants

Lai, H., He, J., Hurtado, J., Stahnke, J., Fuchs, A., Mehlhop, E., Gorlatov, S., Loos, A., Diamond, M. S. & Chen, Q. 2014 In : Plant Biotechnology Journal. 12, 8, p. 1098-1107 10 p.

Research output: Contribution to journalArticle

West Nile virus
monoclonal antibodies
Monoclonal Antibodies
glycosylation
Glycosylation
2013
52 Citations

Efficient agroinfiltration of plants for high-level transient expression of recombinant proteins

Leuzinger, K., Dent, M., Hurtado, J., Stahnke, J., Lai, H., Zhou, X. & Chen, Q. 2013 In : Journal of visualized experiments : JoVE. 77

Research output: Contribution to journalArticle

Recombinant proteins
Syringes
Recombinant Proteins
Proteins
Infiltration
45 Citations

Plant-derived virus-like particles as vaccines

Chen, Q. & Lai, H. Jan 2013 In : Human Vaccines and Immunotherapeutics. 9, 1, p. 26-49 24 p.

Research output: Contribution to journalArticle

Virus-Like Particle Vaccines
Plant Viruses
Virion
Vaccines
Safety
8 Citations

Virus-like particle vaccines for norovirus gastroenteritis

Chen, Q. Jan 1 2013 Molecular Vaccines: From Prophylaxis to Therapy-Volume 1. Springer-Verlag Vienna, p. 153-181 29 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Virus-Like Particle Vaccines
Norovirus
Gastroenteritis
Virion
Vaccines
2012
21 Citations
West Nile virus
Viruses
Costs and Cost Analysis
Costs
Scalability
62 Citations
Norwalk virus
bioprocessing
virus-like particles
coat proteins
manufacturing
48 Citations

Robust production of virus-like particles and monoclonal antibodies with geminiviral replicon vectors in lettuce

Lai, H., He, J., Engle, M., Diamond, M. S. & Chen, Q. Jan 2012 In : Plant Biotechnology Journal. 10, 1, p. 95-104 10 p.

Research output: Contribution to journalArticle

biopharmaceuticals
replicon
Lettuce
Replicon
virus-like particles
2011
40 Citations

A nonreplicating subunit vaccine protects mice against lethal Ebola virus challenge

Phoolcharoen, W., Dye, J. M., Kilbourne, J., Piensook, K., Pratt, W. D., Arntzen, C. J., Chen, Q., Mason, H. S. & Herbst-Kralovetz, M. M. Dec 20 2011 In : Proceedings of the National Academy of Sciences of the United States of America. 108, 51, p. 20695-20700 6 p.

Research output: Contribution to journalArticle

Ebolavirus
Subunit Vaccines
Antigen-Antibody Complex
Ebola Hemorrhagic Fever
Ebola Vaccines
56 Citations

Expression of an immunogenic Ebola immune complex in Nicotiana benthamiana

Phoolcharoen, W., Bhoo, S. H., Lai, H., Ma, J., Arntzen, C. J., Chen, Q. & Mason, H. S. Sep 2011 In : Plant Biotechnology Journal. 9, 7, p. 807-816 10 p.

Research output: Contribution to journalArticle

antigen-antibody complex
Ebolavirus
Nicotiana benthamiana
Antigen-Antibody Complex
Tobacco
61 Citations

Geminiviral vectors based on bean yellow dwarf virus for production of vaccine antigens and monoclonal antibodies in plants

Chen, Q., He, J., Phoolcharoen, W. & Mason, H. S. Mar 2011 In : Human Vaccines. 7, 3, p. 331-338 8 p.

Research output: Contribution to journalArticle

Vaccines
Monoclonal Antibodies
Viruses
Antigens
Replicon
West Nile virus
Blood-Brain Barrier
Directories
Virus Diseases
Plantibodies
12 Citations

Turning a new leaf

Chen, Q. S. Jun 2011 In : EBR - European Biopharmaceutical Review. SUMMER 2011

Research output: Contribution to journalArticle

2010
98 Citations

High-level rapid production of full-size monoclonal antibodies in plants by a single-vector DNA replicon system

Huang, Z., Phoolcharoen, W., Lai, H., Piensook, K., Cardineau, G., Zeitlin, L., Whaley, K. J., Arntzen, C. J., Mason, H. S. & Chen, Q. May 1 2010 In : Biotechnology and Bioengineering. 106, 1, p. 9-17 9 p.

Research output: Contribution to journalArticle

Replicon
Monoclonal antibodies
Viruses
DNA
Monoclonal Antibodies
66 Citations

Monoclonal antibody produced in plants efficiently treats West Nile virus infection in mice

Laia, H., Engleb, M., Fuchsb, A., Kellera, T., Johnsonc, S., Gorlatovc, S., Diamond, M. S. & Chena, Q. Feb 9 2010 In : Proceedings of the National Academy of Sciences of the United States of America. 107, 6, p. 2419-2424 6 p.

Research output: Contribution to journalArticle

West Nile virus
Virus Diseases
Monoclonal Antibodies
Emerging Communicable Diseases
Viral Fusion Proteins
85 Citations

Norwalk virus-like particles as vaccines

Herbst-Kralovetz, M., Mason, H. S. & Chen, Q. 2010 In : Expert Review of Vaccines. 9, 3, p. 299-307 9 p.

Research output: Contribution to journalArticle

Virus-Like Particle Vaccines
Norwalk virus
Virion
Norovirus
Plant Cells
2009
95 Citations

A DNA replicon system for rapid high-level production of virus-like particles in plants

Huang, Z., Chen, Q., Hjelm, B., Arntzen, C. & Mason, H. Jul 1 2009 In : Biotechnology and Bioengineering. 103, 4, p. 706-714 9 p.

Research output: Contribution to journalArticle

Replicon
Viruses
Virion
DNA
Virus-Like Particle Vaccines
1 Citations

cGMP processing of a plant-produced human vaccine candidate for sexually transmitted infections

Chen, Q., Morris, G. & Lai, H. 2009 American Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009. Vol. 1, p. 468-473 6 p.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

sexually transmitted diseases
good manufacturing practices
Sexually Transmitted Diseases
Vaccines
vaccines
124 Citations

Genome sequences of three agrobacterium biovars help elucidate the evolution of multichromosome genomes in bacteria

Slater, S. C. , Goldman, B. S. , Goodner, B. , Setubal, J. C. , Farrand, S. K. , Nester, E. W. , Burr, T. J. , Banta, L. , Dickerman, A. W. , Paulsen, I. , Otten, L. , Suen, G. , Welch, R. , Almeida, N. F. , Arnold, F. , Burton, O. T. , Du, Z. , Ewing, A. , Godsy, E. , Heisel, S. & 16 others Houmiel, K. L., Jhaveri, J., Lu, J., Miller, N. M., Norton, S., Chen, Q., Phoolcharoen, W., Ohlin, V., Ondrusek, D., Pride, N., Stricklin, S. L., Sun, J., Wheeler, C., Wilson, L., Zhu, H. & Wood, D. W. Apr 2009 In : Journal of Bacteriology. 191, 8, p. 2501-2511 11 p.

Research output: Contribution to journalArticle

Agrobacterium
Chromosomes
Genome
Bacteria
Rhizobiaceae
West Nile virus
Monoclonal Antibodies
Infection
Antibodies
Virus Diseases
2008
117 Citations

An efficient plant viral expression system generating orally immunogenic Norwalk virus-like particles

Santi, L., Batchelor, L., Huang, Z., Hjelm, B., Kilbourne, J., Arntzen, C. J., Chen, Q. & Mason, H. S. Mar 28 2008 In : Vaccine. 26, 15, p. 1846-1854 9 p.

Research output: Contribution to journalArticle

Norwalk virus
virus-like particles
Virion
oral vaccination
Immunization
Geminiviridae
replicon
plant proteins
monoclonal antibodies
biopharmaceuticals
130 Citations

Viral vectors for production of recombinant proteins in plants

Lico, C., Chen, Q. & Santi, L. Aug 2008 In : Journal of Cellular Physiology. 216, 2, p. 366-377 12 p.

Research output: Contribution to journalArticle

Recombinant Proteins
Plant Pathology
Genetic engineering
Molecular biology
Genetic Engineering
2005
5 Citations

Expression of porcine prorelaxin in transgenic tobacco

Buswell, S., Medina-Bolivar, F., Chen, Q., Van Cott, K. & Zhang, C. 2005 In : Annals of the New York Academy of Sciences. 1041, p. 77-81 5 p.

Research output: Contribution to journalArticle

Relaxin
Tobacco
Swine
Genetically Modified Plants
Polymerase chain reaction
1996
63 Citations

Isolation and characterization of glutamine synthetase genes in Chlamydomonas reinhardtii

Chen, Q. & Silflow, C. D. Nov 1996 In : Plant Physiology. 112, 3, p. 987-996 10 p.

Research output: Contribution to journalArticle

Chlamydomonas reinhardtii
Glutamate-Ammonia Ligase
glutamate-ammonia ligase
assimilation (physiology)
Complementary DNA
1994
43 Citations

A 21-kDa chloroplast heat shock protein assembles into high molecular weight complexes in vivo and in organelle

Chen, Q., Osteryoung, K. & Vierling, E. May 6 1994 In : Journal of Biological Chemistry. 269, 18, p. 13216-13223 8 p.

Research output: Contribution to journalArticle

Chloroplast Proteins
Chloroplasts
Heat-Shock Proteins
Organelles
Molecular Weight
1991
77 Citations

Analysis of conserved domains identifies a unique structural feature of a chloroplast heat shock protein

Chen, Q. & Vierling, E. May 1991 In : MGG Molecular & General Genetics. 226, 3, p. 425-431 7 p.

Research output: Contribution to journalArticle

Chloroplast Proteins
Chloroplasts
Heat-Shock Proteins
Methionine
Petunia
5 Citations

Analysis of conserved domains identifies a unique structural feature of a chloroplast heat shock protein.

Chen, Q. & Vierling, E. Aug 1991 In : Molecular and General Genetics. 228, 1-2, p. 328 1 p.

Research output: Contribution to journalArticle

Nucleic Acid Sequence Homology
Molecular Sequence Data
Chloroplast Proteins
Brassica
Chloroplasts

Erratum

Chen, Q. & Vierling, E. Aug 1991 In : MGG Molecular & General Genetics. 228, 1-2, p. 328 1 p.

Research output: Contribution to journalArticle

1990
75 Citations

Accumulation, stability, and localization of a major chloroplast heat-shock protein

Chen, Q., Lauzon, L. M., DeRocher, A. E. & Vierling, E. Jun 1990 In : Journal of Cell Biology. 110, 6, p. 1873-1883 11 p.

Research output: Contribution to journalArticle

Chloroplast Proteins
Heat-Shock Proteins
Chloroplasts
Molecular Weight
Peas
1989
39 Citations

The major low-molecular-weight heat shock protein in chloroplasts shows antigenic conservation among diverse higher plant species.

Vierling, E., Harris, L. M. & Chen, Q. Feb 1989 In : Molecular and Cellular Biology. 9, 2, p. 461-468 8 p.

Research output: Contribution to journalArticle

Chloroplasts
Heat-Shock Proteins
Molecular Weight
Chloroplast Proteins
Peas