Petra Fromme

Regents Professor

  • 14934 Citations
  • 54 h-Index
1986 …2021
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Research Output 1986 2019

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Article
2019
1 Citation (Scopus)

High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source

Martin-Garcia, J. M., Zhu, L., Mendez, D., Lee, M. Y., Chun, E., Li, C., Hu, H., Subramanian, G., Kissick, D., Ogata, C., Henning, R., Ishchenko, A., Dobson, Z., Zhang, S., Weierstall, U., Spence, J., Fromme, P., Zatsepin, N., Fischetti, R. F., Cherezov, V. & 1 others, Liu, W., Jan 1 2019, In : IUCrJ. 6, p. 412-425 14 p.

Research output: Contribution to journalArticle

Open Access
Crystallography
Microcrystals
Synchrotrons
microcrystals
Synchrotron radiation
3 Citations (Scopus)

Snapshot of an oxygen intermediate in the catalytic reaction of cytochrome c oxidase

Ishigami, I., Lewis-Ballester, A., Echelmeier, A., Brehm, G., Zatsepin, N., Grant, T. D., Coe, J. D., Lisova, S., Nelson, G., Zhang, S., Dobson, Z. F., Boutet, S., Sierra, R. G., Batyuk, A., Fromme, P., Fromme, R., Spence, J., Ros, A., Yeh, S. R. & Rousseau, D. L., Feb 26 2019, In : Proceedings of the National Academy of Sciences of the United States of America. 116, 9, p. 3572-3577 6 p.

Research output: Contribution to journalArticle

Electron Transport Complex IV
Oxygen
Protons
Protein Conformation
Crystallography

The Catalytic Cycle of Water Oxidation in Crystallized Photosystem II Complexes: Performance and Requirements for Formation of Intermediates

Ananyev, G., Roy-Chowdhury, S., Gates, C., Fromme, P. & Dismukes, G. C., Feb 1 2019, In : ACS Catalysis. 9, 2, p. 1396-1407 12 p.

Research output: Contribution to journalArticle

Photosystem II Protein Complex
Oxidation
Crystals
Electrons
Water
1 Citation (Scopus)

X-ray Emission Spectroscopy at X-ray Free Electron Lasers: Limits to Observation of the Classical Spectroscopic Response for Electronic Structure Analysis

Jensen, S. C., Sullivan, B., Hartzler, D. A., Aguilar, J. M., Awel, S., Bajt, S., Basu, S., Bean, R., Chapman, H. N., Conrad, C., Frank, M., Fromme, R., Martin-Garcia, J. M., Grant, T. D., Heymann, M., Hunter, M. S., Ketawala, G., Kirian, R., Knoska, J., Kupitz, C. & 23 others, Li, X., Liang, M., Lisova, S., Mariani, V., Mazalova, V., Messerschmidt, M., Moran, M., Nelson, G., Oberthür, D., Schaffer, A., Sierra, R. G., Vaughn, N., Weierstall, U., Wiedorn, M. O., Xavier, P. L., Yang, J. H., Yefanov, O., Zatsepin, N., Aquila, A., Fromme, P., Boutet, S., Seidler, G. T. & Pushkar, Y., Feb 7 2019, In : Journal of Physical Chemistry Letters. 10, 3, p. 441-446 6 p.

Research output: Contribution to journalArticle

X ray lasers
Free electron lasers
free electron lasers
Electronic structure
electronic structure
2018
21 Citations (Scopus)

Enzyme intermediates captured "on the fly" by mix-and-inject serial crystallography

Olmos, J. L., Pandey, S., Martin-Garcia, J. M., Calvey, G., Katz, A., Knoska, J., Kupitz, C., Hunter, M. S., Liang, M., Oberthuer, D., Yefanov, O., Wiedorn, M., Heyman, M., Holl, M., Pande, K., Barty, A., Miller, M. D., Stern, S., Roy-Chowdhury, S., Coe, J. & 30 others, Nagaratnam, N., Zook, J., Verburgt, J., Norwood, T., Poudyal, I., Xu, D., Koglin, J., Seaberg, M. H., Zhao, Y., Bajt, S., Grant, T., Mariani, V., Nelson, G., Subramanian, G., Bae, E., Fromme, R., Fung, R., Schwander, P., Frank, M., White, T. A., Weierstall, U., Zatsepin, N., Spence, J., Fromme, P., Chapman, H. N., Pollack, L., Tremblay, L., Ourmazd, A., Phillips, G. N. & Schmidt, M., May 31 2018, In : BMC Biology. 16, 1, 59.

Research output: Contribution to journalArticle

crystallography
Crystallography
Diptera
X-radiation
X-Rays

Free-electron laser data for multiple-particle fluctuation scattering analysis

Pande, K., Donatelli, J. J., Malmerberg, E., Foucar, L., Poon, B. K., Sutter, M., Botha, S., Basu, S., Doak, R. B., Dörner, K., Epp, S. W., Englert, L., Fromme, R., Hartmann, E., Hartmann, R., Hauser, G., Hattne, J., Hosseinizadeh, A., Kassemeyer, S., Lomb, L. & 16 others, Carron Montero, S. F., Menzel, A., Rolles, D., Rudenko, A., Seibert, M. M., Sierra, R. G., Schwander, P., Ourmazd, A., Fromme, P., Sauter, N. K., Bogan, M., Bozek, J., Bostedt, C., Schlichting, I., Kerfeld, C. A. & Zwart, P. H., Jan 1 2018, In : Scientific data. 5, 180201.

Research output: Contribution to journalArticle

Free Electron Laser
Free electron lasers
Diffraction patterns
fluctuation
Scattering
2 Citations (Scopus)

Generation of High-Specificity Antibodies against Membrane Proteins Using DNA-Gold Micronanoplexes for Gene Gun Immunization

Hansen, D., Craciunescu, F. M., Fromme, P., Johnston, S. & Sykes, K. F., Feb 21 2018, In : Current Protocols in Protein Science. 91, p. 29.20.1-29.20.22

Research output: Contribution to journalArticle

Immunization
Antibody Specificity
Firearms
Gold
Membrane Proteins

Gordon Research Conference on photosynthesis: photosynthetic plasticity from the environment to synthetic systems

Gisriel, C., Saroussi, S., Ramundo, S., Fromme, P. & Govindjee, Jan 2 2018, (Accepted/In press) In : Photosynthesis Research. p. 1-13 13 p.

Research output: Contribution to journalArticle

Photosynthesis
Plasticity
photosynthesis
Research
Gift Giving
27 Citations (Scopus)

Megahertz serial crystallography

Wiedorn, M. O., Oberthür, D., Bean, R., Schubert, R., Werner, N., Abbey, B., Aepfelbacher, M., Adriano, L., Allahgholi, A., Al-Qudami, N., Andreasson, J., Aplin, S., Awel, S., Ayyer, K., Bajt, S., Barák, I., Bari, S., Bielecki, J., Botha, S., Boukhelef, D. & 106 others, Brehm, W., Brockhauser, S., Cheviakov, I., Coleman, M. A., Cruz-Mazo, F., Danilevski, C., Darmanin, C., Doak, R. B., Domaracky, M., Dörner, K., Du, Y., Fangohr, H., Fleckenstein, H., Frank, M., Fromme, P., Gañán-Calvo, A. M., Gevorkov, Y., Giewekemeyer, K., Ginn, H. M., Graafsma, H., Graceffa, R., Greiffenberg, D., Gumprecht, L., Göttlicher, P., Hajdu, J., Hauf, S., Heymann, M., Holmes, S., Horke, D. A., Hunter, M. S., Imlau, S., Kaukher, A., Kim, Y., Klyuev, A., Knoška, J., Kobe, B., Kuhn, M., Kupitz, C., Küpper, J., Lahey-Rudolph, J. M., Laurus, T., Le Cong, K., Letrun, R., Xavier, P. L., Maia, L., Maia, F. R. N. C., Mariani, V., Messerschmidt, M., Metz, M., Mezza, D., Michelat, T., Mills, G., Monteiro, D. C. F., Morgan, A., Mühlig, K., Munke, A., Münnich, A., Nette, J., Nugent, K. A., Nuguid, T., Orville, A. M., Pandey, S., Pena, G., Villanueva-Perez, P., Poehlsen, J., Previtali, G., Redecke, L., Riekehr, W. M., Rohde, H., Round, A., Safenreiter, T., Sarrou, I., Sato, T., Schmidt, M., Schmitt, B., Schönherr, R., Schulz, J., Sellberg, J. A., Seibert, M. M., Seuring, C., Shelby, M. L., Shoeman, R. L., Sikorski, M., Silenzi, A., Stan, C. A., Shi, X., Stern, S., Sztuk-Dambietz, J., Szuba, J., Tolstikova, A., Trebbin, M., Trunk, U., Vagovic, P., Ve, T., Weinhausen, B., White, T. A., Wrona, K., Xu, C., Yefanov, O., Zatsepin, N., Zhang, J., Perbandt, M., Mancuso, A. P., Betzel, C., Chapman, H. & Barty, A., Dec 1 2018, In : Nature Communications. 9, 1, 4025.

Research output: Contribution to journalArticle

X ray lasers
Crystallography
crystallography
Pulse repetition rate
Free electron lasers
9 Citations (Scopus)

Rapid sample delivery for megahertz serial crystallography at X-ray FELs

Wiedorn, M. O., Awel, S., Morgan, A. J., Ayyer, K., Gevorkov, Y., Fleckenstein, H., Roth, N., Adriano, L., Bean, R., Beyerlein, K. R., Chen, J., Coe, J., Cruz-Mazo, F., Ekeberg, T., Graceffa, R., Heymann, M., Horke, D. A., Knoška, J., Mariani, V., Nazari, R. & 18 others, Oberthür, D., Samanta, A. K., Sierra, R. G., Stan, C. A., Yefanov, O., Rompotis, D., Correa, J., Erk, B., Treusch, R., Schulz, J., Hogue, B., Gañán-Calvo, A. M., Fromme, P., Küpper, J., Rode, A. V., Bajt, S., Kirian, R. & Chapman, H. N., Jan 1 2018, In : IUCrJ. 5, p. 574-584 11 p.

Research output: Contribution to journalArticle

X ray lasers
Crystallography
Free electron lasers
X Ray Crystallography
free electron lasers
3 Citations (Scopus)

Solving protein structure from sparse serial microcrystal diffraction data at a storage-ring synchrotron source

Lan, T. Y., Wierman, J. L., Tate, M. W., Philipp, H. T., Martin-Garcia, J. M., Zhu, L., Kissick, D., Fromme, P., Fischetti, R. F., Liu, W., Elser, V. & Gruner, S. M., Jan 1 2018, In : IUCrJ. 5, p. 548-558 11 p.

Research output: Contribution to journalArticle

Microcrystals
Synchrotrons
Storage rings
microcrystals
synchrotrons
3 Citations (Scopus)

Structural basis for light control of cell development revealed by crystal structures of a myxobacterial phytochrome

Woitowich, N. C., Halavaty, A. S., Waltz, P., Kupitz, C., Valera, J., Tracy, G., Gallagher, K. D., Claesson, E., Nakane, T., Pandey, S., Nelson, G., Tanaka, R., Nango, E., Mizohata, E., Owada, S., Tono, K., Joti, Y., Nugent, A. C., Patel, H., Mapara, A. & 21 others, Hopkins, J., Duong, P., Bizhga, D., Kovaleva, S. E., Peter, R. S., Hernandez, C. N., Ozarowski, W. B., Roy-Chowdhuri, S., Yang, J. H., Edlund, P., Takala, H., Ihalainen, J., Brayshaw, J., Norwood, T., Poudyal, I., Fromme, P., Spence, J., Moffat, K., Westenhoff, S., Schmidt, M. & Stojković, E. A., Jan 1 2018, In : IUCrJ. 5, p. 619-634 16 p.

Research output: Contribution to journalArticle

phytochrome
Phytochrome
Threonine
Chromophores
Myxococcales

Supersaturation-controlled microcrystallization and visualization analysis for serial femtosecond crystallography

Lee, D. B., Kim, J. M., Seok, J. H., Lee, J. H., Jo, J. D., Mun, J. Y., Conrad, C., Coe, J., Nelson, G., Hogue, B., White, T. A., Zatsepin, N., Weierstall, U., Barty, A., Chapman, H., Fromme, P., Spence, J., Chung, M. S., Oh, C. H. & Kim, K. H., Dec 1 2018, In : Scientific Reports. 8, 1, 2541.

Research output: Contribution to journalArticle

supersaturation
crystallography
microcrystals
free electron lasers
evaporation
2017
29 Citations (Scopus)

Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser

Gati, C., Oberthuer, D., Yefanov, O., Bunker, R. D., Stellato, F., Chiu, E., Yeh, S. M., Aquila, A., Basu, S., Bean, R., Beyerlein, K. R., Botha, S., Boutet, S., DePonte, D. P., Doak, R. B., Fromme, R., Galli, L., Grotjohann, I., James, D. R., Kupitz, C. & 17 others, Lomb, L., Messerschmidt, M., Nass, K., Rendek, K., Shoeman, R. L., Wang, D., Weierstall, U., White, T. A., Williams, G. J., Zatsepin, N., Fromme, P., Spence, J., Goldie, K. N., Jehle, J. A., Metcalf, P., Barty, A. & Chapman, H. N., Feb 28 2017, In : Proceedings of the National Academy of Sciences of the United States of America. 114, 9, p. 2247-2252 6 p.

Research output: Contribution to journalArticle

Granulovirus
Lasers
X-Rays
Electrons
Radiation
4 Citations (Scopus)

Bacterial expression, correct membrane targeting and functional folding of the HIV-1 membrane protein Vpu using a periplasmic signal peptide

Deb, A., Johnson, W. A., Kline, A. P., Scott, B. J., Meador, L. R., Srinivas, D., Martin-Garcia, J. M., Dörner, K., Borges, C., Misra, R., Hogue, B., Fromme, P. & Leket-Mor, T., Feb 1 2017, In : PLoS One. 12, 2, e0172529.

Research output: Contribution to journalArticle

viral proteins
Viral Proteins
Protein Sorting Signals
Human immunodeficiency virus 1
signal peptide
17 Citations (Scopus)

Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source

Reddy, H. K. N., Yoon, C. H., Aquila, A., Awel, S., Ayyer, K., Barty, A., Berntsen, P., Bielecki, J., Bobkov, S., Bucher, M., Carini, G. A., Carron, S., Chapman, H., Daurer, B., Demirci, H., Ekeberg, T., Fromme, P., Hajdu, J., Hanke, M. F., Hart, P. & 25 others, Hogue, B., Hosseinizadeh, A., Kim, Y., Kirian, R., Kurta, R. P., Larsson, D. S. D., Duane Loh, N., Maia, F. R. N. C., Mancuso, A. P., Mühlig, K., Munke, A., Nam, D., Nettelblad, C., Ourmazd, A., Rose, M., Schwander, P., Seibert, M., Sellberg, J. A., Song, C., Spence, J., Svenda, M., Van Der Schot, G., Vartanyants, I. A., Williams, G. J. & Lourdu Xavier, P., Jun 27 2017, In : Scientific data. 4, 170079.

Research output: Contribution to journalArticle

X ray lasers
Molecular imaging
Soft X-ray
Free electron lasers
Viruses
28 Citations (Scopus)

Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses

Kurta, R. P., Donatelli, J. J., Yoon, C. H., Berntsen, P., Bielecki, J., Daurer, B. J., Demirci, H., Fromme, P., Hantke, M. F., Maia, F. R. N. C., Munke, A., Nettelblad, C., Pande, K., Reddy, H. K. N., Sellberg, J. A., Sierra, R. G., Svenda, M., Van Der Schot, G., Vartanyants, I. A., Williams, G. J. & 4 others, Xavier, P. L., Aquila, A., Zwart, P. H. & Mancuso, A. P., Oct 12 2017, In : Physical Review Letters. 119, 15, 158102.

Research output: Contribution to journalArticle

viruses
x ray lasers
angular correlation
free electron lasers
pulses
9 Citations (Scopus)

Crystal structure of CO-bound cytochrome c oxidase determined by serial femtosecond X-ray crystallography at room temperature

Ishigami, I., Zatsepin, N., Hikita, M., Conrad, C. E., Nelson, G., Coe, J. D., Basu, S., Grant, T. D., Seaberg, M. H., Sierra, R. G., Hunter, M. S., Fromme, P., Fromme, R., Yeh, S. R. & Rousseau, D. L., Jul 25 2017, In : Proceedings of the National Academy of Sciences of the United States of America. 114, 30, p. 8011-8016 6 p.

Research output: Contribution to journalArticle

X Ray Crystallography
Electron Transport Complex IV
Carbon Monoxide
Synchrotrons
Temperature
3 Citations (Scopus)

Diffraction data of core-shell nanoparticles from an X-ray free electron laser

Li, X., Chiu, C. Y., Wang, H. J., Kassemeyer, S., Botha, S., Shoeman, R. L., Lawrence, R. M., Kupitz, C., Kirian, R., James, D., Wang, DI., Nelson, G., Messerschmidt, M., Boutet, S., Williams, G. J., Hartmann, E., Jafarpour, A., Foucar, L. M., Barty, A., Chapman, H. & 13 others, Liang, M., Menzel, A., Wang, F., Basu, S., Fromme, R., Doak, R. B., Fromme, P., Weierstall, U., Huang, M. H., Spence, J., Schlichting, I., Hogue, B. & Liu, H., Apr 11 2017, In : Scientific data. 4, 170048.

Research output: Contribution to journalArticle

X ray lasers
X-ray Laser
Free Electron Laser
Free electron lasers
Nanoparticles
31 Citations (Scopus)

Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation

Martin-Garcia, J. M., Conrad, C. E., Nelson, G., Stander, N., Zatsepin, N., Zook, J., Zhu, L., Geiger, J., Chun, E., Kissick, D., Hilgart, M. C., Ogata, C., Ishchenko, A., Nagaratnam, N., Roy-Chowdhury, S., Coe, J., Subramanian, G., Schaffer, A., James, D., Ketwala, G. & 10 others, Venugopalan, N., Xu, S., Corcoran, S., Ferguson, D., Weierstall, U., Spence, J., Cherezov, V., Fromme, P., Fischetti, R. F. & Liu, W., 2017, In : IUCrJ. 4, p. 439-454 16 p.

Research output: Contribution to journalArticle

Crystallography
Microcrystals
Synchrotrons
microcrystals
Phycocyanin
1 Citation (Scopus)

Split-Second Reactions

Fromme, P. & Spence, J., Apr 18 2017, In : Scientific American. 316, 5, p. 62-67 6 p.

Research output: Contribution to journalArticle

36 Citations (Scopus)

Structural enzymology using X-ray free electron lasers

Kupitz, C., Olmos, J. L., Holl, M., Tremblay, L., Pande, K., Pandey, S., Oberthür, D., Hunter, M., Liang, M., Aquila, A., Tenboer, J., Calvey, G., Katz, A., Chen, Y., Wiedorn, M. O., Knoska, J., Meents, A., Majriani, V., Norwood, T., Poudyal, I. & 30 others, Grant, T., Miller, M. D., Xu, W., Tolstikova, A., Morgan, A., Metz, M., Martin-Gracia, J., Zook, J. D., Roy-Chowdhury, S., Coe, J., Nagaratnam, N., Meza, D., Fromme, R., Basu, S., Frank, M., White, T., Barty, A., Bajt, S., Yefanov, O., Chapman, H. N., Zatsepin, N., Nelson, G., Weierstall, U., Spence, J., Schwander, P., Pollack, L., Fromme, P., Ourmazd, A., Phillips, G. N. & Schmidt, M., Jul 1 2017, In : Structural Dynamics. 4, 4, 044003.

Research output: Contribution to journalArticle

enzymology
X ray lasers
Crystallography
Ceftriaxone
Free electron lasers
78 Citations (Scopus)

Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography

Stagno, J. R., Liu, Y., Bhandari, Y. R., Conrad, C. E., Panja, S., Swain, M., Fan, L., Nelson, G., Li, C., Wendel, D. R., White, T. A., Coe, J. D., Wiedorn, M. O., Knoska, J., Oberthuer, D., Tuckey, R. A., Yu, P., Dyba, M., Tarasov, S. G., Weierstall, U. & 18 others, Grant, T. D., Schwieters, C. D., Zhang, J., Ferré-D'Amaré, A. R., Fromme, P., Draper, D. E., Liang, M., Hunter, M. S., Boutet, S., Tan, K., Zuo, X., Ji, X., Barty, A., Zatsepin, N., Chapman, H. N., Spence, J., Woodson, S. A. & Wang, Y. X., Jan 12 2017, In : Nature. 541, 7636, p. 242-246 5 p.

Research output: Contribution to journalArticle

Riboswitch
Crystallography
Lasers
X-Rays
RNA
2016
50 Citations (Scopus)

AXSIS: Exploring the frontiers in attosecond X-ray science, imaging and spectroscopy

Kärtner, F. X., Ahr, F., Calendron, A. L., Çankaya, H., Carbajo, S., Chang, G., Cirmi, G., Dörner, K., Dorda, U., Fallahi, A., Hartin, A., Hemmer, M., Hobbs, R., Hua, Y., Huang, W. R., Letrun, R., Matlis, N., Mazalova, V., Mücke, O. D., Nanni, E. & 17 others, Putnam, W., Ravi, K., Reichert, F., Sarrou, I., Wu, X., Yahaghi, A., Ye, H., Zapata, L., Zhang, D., Zhou, C., Miller, R. J. D., Berggren, K. K., Graafsma, H., Meents, A., Assmann, R. W., Chapman, H. N. & Fromme, P., Jan 13 2016, (Accepted/In press) In : Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment.

Research output: Contribution to journalArticle

Spectroscopy
Imaging techniques
X rays
X ray crystallography
spectroscopy

Characterization of Protein Nanocrystals Based on the Reversibility of Crystallization

Dörner, K., Martin-Garcia, J. M., Kupitz, C., Gong, Z., Mallet, T. C., Chen, L., Wachter, R. & Fromme, P., Jul 6 2016, In : Crystal Growth and Design. 16, 7, p. 3838-3845 8 p.

Research output: Contribution to journalArticle

Crystallization
Nanocrystals
nanocrystals
crystallization
proteins
35 Citations (Scopus)

Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source

Munke, A., Andreasson, J., Aquila, A., Awel, S., Ayyer, K., Barty, A., Bean, R. J., Berntsen, P., Bielecki, J., Boutet, S., Bucher, M., Chapman, H. N., Daurer, B. J., Demirci, H., Elser, V., Fromme, P., Hajdu, J., Hantke, M. F., Higashiura, A., Hogue, B. & 33 others, Hosseinizadeh, A., Kim, Y., Kirian, R., Reddy, H. K. N., Lan, T. Y., Larsson, D. S. D., Liu, H., Loh, N. D., Maia, F. R. N. C., Mancuso, A. P., Mühlig, K., Nakagawa, A., Nam, D., Nelson, G., Nettelblad, C., Okamoto, K., Ourmazd, A., Rose, M., Van Der Schot, G., Schwander, P., Seibert, M. M., Sellberg, J. A., Sierra, R. G., Song, C., Svenda, M., Timneanu, N., Vartanyants, I. A., Westphal, D., Wiedorn, M. O., Williams, G. J., Xavier, P. L., Yoon, C. H. & Zook, J., Aug 1 2016, In : Scientific data. 3, 160064.

Research output: Contribution to journalArticle

Hard X-ray
Viruses
Virus
Light sources
Diffraction
158 Citations (Scopus)

Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein

Pande, K., Hutchison, C. D. M., Groenhof, G., Aquila, A., Robinson, J. S., Tenboer, J., Basu, S., Boutet, S., DePonte, D. P., Liang, M., White, T. A., Zatsepin, N., Yefanov, O., Morozov, D., Oberthuer, D., Gati, C., Subramanian, G., James, D., Zhao, Y., Koralek, J. & 24 others, Brayshaw, J., Kupitz, C., Conrad, C., Roy-Chowdhury, S., Coe, J. D., Metz, M., Xavier, P. L., Grant, T. D., Koglin, J. E., Ketawala, G., Fromme, R., Srajer, V., Henning, R., Spence, J., Ourmazd, A., Schwander, P., Weierstall, U., Frank, M., Fromme, P., Barty, A., Chapman, H. N., Moffat, K., Van Thor, J. J. & Schmidt, M., May 6 2016, In : Science. 352, 6286, p. 725-729 5 p.

Research output: Contribution to journalArticle

Light
Crystallography
Proteins
Photons
X-Rays
5 Citations (Scopus)

Immunological Characterization of Plant-Based HIV-1 Gag/Dgp41 Virus-Like Particles

Kessans, S. A., Linhart, M. D., Meador, L. R., Kilbourne, J., Hogue, B., Fromme, P., Matoba, N. & Leket-Mor, T., 2016, In : PLoS One. 11, 3, p. e0151842

Research output: Contribution to journalArticle

virus-like particles
Human immunodeficiency virus 1
Viruses
Virion
HIV-1
37 Citations (Scopus)

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Nogly, P., Panneels, V., Nelson, G., Gati, C., Kimura, T., Milne, C., Milathianaki, D., Kubo, M., Wu, W., Conrad, C., Coe, J., Bean, R., Zhao, Y., Båth, P., Dods, R., Harimoorthy, R., Beyerlein, K. R., Rheinberger, J., James, D., DePonte, D. & 20 others, Li, C., Sala, L., Williams, G. J., Hunter, M. S., Koglin, J. E., Berntsen, P., Nango, E., Iwata, S., Chapman, H. N., Fromme, P., Frank, M., Abela, R., Boutet, S., Barty, A., White, T. A., Weierstall, U., Spence, J., Neutze, R., Schertler, G. & Standfuss, J., Aug 22 2016, In : Nature Communications. 7, 12314.

Research output: Contribution to journalArticle

Bacteriorhodopsins
Crystallography
injectors
crystallography
delivery
69 Citations (Scopus)

Macromolecular diffractive imaging using imperfect crystals

Ayyer, K., Yefanov, O. M., Oberthür, D., Roy-Chowdhury, S., Galli, L., Mariani, V., Basu, S., Coe, J., Conrad, C. E., Fromme, R., Schaffer, A., Dörner, K., James, D., Kupitz, C., Metz, M., Nelson, G., Xavier, P. L., Beyerlein, K. R., Schmidt, M., Sarrou, I. & 14 others, Spence, J., Weierstall, U., White, T. A., Yang, J. H., Zhao, Y., Liang, M., Aquila, A., Hunter, M. S., Robinson, J. S., Koglin, J. E., Boutet, S., Fromme, P., Barty, A. & Chapman, H. N., Feb 10 2016, In : Nature. 530, 7589, p. 202-206 5 p.

Research output: Contribution to journalArticle

Photosystem II Protein Complex
Crystallography
X Ray Crystallography
Cell Size
X-Ray Diffraction
45 Citations (Scopus)

Native phasing of x-ray free-electron laser data for a G protein–coupled receptor

Batyuk, A., Galli, L., Ishchenko, A., Han, G. W., Gati, C., Popov, P. A., Lee, M. Y., Stauch, B., White, T. A., Barty, A., Aquila, A., Hunter, M. S., Liang, M., Boutet, S., Pu, M., Liu, Z. J., Nelson, G., James, D., Li, C., Zhao, Y. & 7 others, Spence, J., Liu, W., Fromme, P., Katritch, V., Weierstall, U., Stevens, R. C. & Cherezov, V., Sep 1 2016, In : Science advances. 2, 9, e1600292.

Research output: Contribution to journalArticle

Lasers
X-Rays
Electrons
Adenosine A2A Receptors
Crystallography
5 Citations (Scopus)

Polyclonal antibody production for membrane proteins via genetic immunization

Hansen, D., Robida, M. D., Craciunescu, F. M., Loskutov, A. V., Dörner, K., Rodenberry, J. C., Wang, X., Olson, T. L., Patel, H., Fromme, P. & Sykes, K. F., Feb 24 2016, In : Scientific Reports. 6, 21925.

Research output: Contribution to journalArticle

Antibody Formation
Immunization
Membrane Proteins
Antibodies
Francisella tularensis
10 Citations (Scopus)

Protein Crystallization in an Actuated Microfluidic Nanowell Device

Abdallah, B. G., Roy-Chowdhury, S., Fromme, R., Fromme, P. & Ros, A., Apr 6 2016, In : Crystal Growth and Design. 16, 4, p. 2074-2082 9 p.

Research output: Contribution to journalArticle

microfluidic devices
Crystallization
Microfluidics
crystallization
proteins

Second harmonic generation correlation spectroscopy for characterizing translationally diffusing protein nanocrystals

Dow, X. Y., Dettmar, C. M., DeWalt, E. L., Newman, J. A., Dow, A. R., Roy-Chowdhury, S., Coe, J. D., Kupitz, C., Fromme, P. & Simpson, G. J., 2016, In : Acta Crystallographica Section D: Structural Biology. 72, 7, p. 849-859 11 p.

Research output: Contribution to journalArticle

Nanoparticles
Spectrum Analysis
Proteins
Optical Phenomena
Photosystem I Protein Complex
38 Citations (Scopus)

Serial femtosecond crystallography: A revolution in structural biology

Martin-Garcia, J. M., Conrad, C. E., Coe, J., Roy-Chowdhury, S. & Fromme, P., Jul 15 2016, In : Archives of Biochemistry and Biophysics. 602, p. 32-47 16 p.

Research output: Contribution to journalArticle

Crystallography
Radiation damage
X-Rays
Radiation
X ray lasers

Serial femtosecond crystallography opens new avenues for structural biology

Coe, J. & Fromme, P., Mar 1 2016, In : Protein and Peptide Letters. 23, 3, p. 255-272 18 p.

Research output: Contribution to journalArticle

Crystallography
Free electron lasers
Lasers
X-Rays
Electrons
1 Citation (Scopus)

Spectral Hole Burning in Cyanobacterial Photosystem i with P700 in Oxidized and Neutral States

Herascu, N., Hunter, M. S., Shafiei, G., Najafi, M., Johnson, T. W., Fromme, P. & Zazubovich, V., Oct 13 2016, In : Journal of Physical Chemistry B. 120, 40, p. 10483-10495 13 p.

Research output: Contribution to journalArticle

Photosystem I Protein Complex
hole burning
Pigments
pigments
Recovery
16 Citations (Scopus)

The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography

Edlund, P., Takala, H., Claesson, E., Henry, L., Dods, R., Lehtivuori, H., Panman, M., Pande, K., White, T., Nakane, T., Berntsson, O., Gustavsson, E., Båth, P., Modi, V., Roy-Chowdhury, S., Zook, J., Berntsen, P., Pandey, S., Poudyal, I., Tenboer, J. & 16 others, Kupitz, C., Barty, A., Fromme, P., Koralek, J. D., Tanaka, T., Spence, J., Liang, M., Hunter, M. S., Boutet, S., Nango, E., Moffat, K., Groenhof, G., Ihalainen, J., Stojkovi, E. A., Schmidt, M. & Westenhoff, S., Oct 19 2016, In : Scientific Reports. 6, 35279.

Research output: Contribution to journalArticle

Phytochrome
Bacterial Structures
Crystallography
Temperature
Deinococcus
19 Citations (Scopus)

X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex

Zhou, X. E., Gao, X., Barty, A., Kang, Y., He, Y., Liu, W., Ishchenko, A., White, T. A., Yefanov, O., Han, G. W., Xu, Q., De Waal, P. W., Suino-Powell, K. M., Boutet, S., Williams, G. J., Wang, M., Li, D., Caffrey, M., Chapman, H. N., Spence, J. & 6 others, Fromme, P., Weierstall, U., Stevens, R. C., Cherezov, V., Melcher, K. & Xu, H. E., Apr 12 2016, In : Scientific data. 3, 201621.

Research output: Contribution to journalArticle

X ray lasers
X-ray Laser
Free Electron Laser
Free electron lasers
Femtosecond
2015
62 Citations (Scopus)

A novel inert crystal delivery medium for serial femtosecond crystallography

Conrad, C. E., Basu, S., James, D., Wang, D., Schaffer, A., Roy-Chowdhury, S., Zatsepin, N., Aquila, A., Coe, J., Gati, C., Hunter, M. S., Koglin, J. E., Kupitz, C., Nelson, G., Subramanian, G., White, T. A., Zhao, Y., Zook, J., Boutet, S., Cherezov, V. & 4 others, Spence, J., Fromme, R., Weierstall, U. & Fromme, P., Jul 1 2015, In : IUCrJ. 2, p. 421-430 10 p.

Research output: Contribution to journalArticle

Crystallography
crystallography
delivery
Sepharose
proteins
1 Citation (Scopus)

Biophysical characterization of a vaccine candidate against HIV-1: The transmembrane and membrane proximal domains of HIV-1 gp41 as a maltose binding protein fusion

Gong, Z., Martin-Garcia, J. M., Daskalova, S. M., Craciunescu, F. M., Song, L., Dörner, K., Hansen, D., Yang, J. H., LaBaer, J., Hogue, B., Leket-Mor, T. & Fromme, P., Aug 21 2015, In : PLoS One. 10, 8, e0136507.

Research output: Contribution to journalArticle

Maltose-Binding Proteins
maltose
Human immunodeficiency virus 1
HIV-1
binding proteins
8 Citations (Scopus)

Critical assessment of the emission spectra of various photosystem II core complexes

Chen, J., Kell, A., Acharya, K., Kupitz, C., Fromme, P. & Jankowiak, R., Apr 2 2015, In : Photosynthesis Research. 124, 3, p. 253-265 13 p.

Research output: Contribution to journalArticle

Photosystem II Protein Complex
photosystem II
Spinacia oleracea
spinach
Electron energy levels
5 Citations (Scopus)

Crystallization of photosystem II for time-resolved structural studies using an X-ray free electron laser

Coe, J., Kupitz, C., Basu, S., Conrad, C. E., Roy-Chowdhury, S., Fromme, R. & Fromme, P., 2015, In : Methods in Enzymology. 557, p. 459-482 24 p.

Research output: Contribution to journalArticle

X ray lasers
Photosystem II Protein Complex
Free electron lasers
Crystallization
Lasers
359 Citations (Scopus)

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser

Kang, Y., Zhou, X. E., Gao, X., He, Y., Liu, W., Ishchenko, A., Barty, A., White, T. A., Yefanov, O., Han, G. W., Xu, Q., De Waal, P. W., Ke, J., Tan, M. H. E., Zhang, C., Moeller, A., West, G. M., Pascal, B. D., Van Eps, N., Caro, L. N. & 52 others, Vishnivetskiy, S. A., Lee, R. J., Suino-Powell, K. M., Gu, X., Pal, K., Ma, J., Zhi, X., Boutet, S., Williams, G. J., Messerschmidt, M., Gati, C., Zatsepin, N., Wang, D., James, D., Basu, S., Roy-Chowdhury, S., Conrad, C. E., Coe, J., Liu, H., Lisova, S., Kupitz, C., Grotjohann, I., Fromme, R., Jiang, Y., Tan, M., Yang, H., Li, J., Wang, M., Zheng, Z., Li, D., Howe, N., Zhao, Y., Standfuss, J., Diederichs, K., Dong, Y., Potter, C. S., Carragher, B., Caffrey, M., Jiang, H., Chapman, H. N., Spence, J., Fromme, P., Weierstall, U., Ernst, O. P., Katritch, V., Gurevich, V. V., Griffin, P. R., Hubbell, W. L., Stevens, R. C., Cherezov, V., Melcher, K. & Xu, H. E., Jul 30 2015, In : Nature. 523, 7562, p. 561-567 7 p.

Research output: Contribution to journalArticle

Arrestin
Rhodopsin
Lasers
X-Rays
G-Protein-Coupled Receptors
13 Citations (Scopus)

High throughput protein nanocrystal fractionation in a microfluidic sorter

Abdallah, B. G., Roy-Chowdhury, S., Coe, J., Fromme, P. & Ros, A., Apr 21 2015, In : Analytical Chemistry. 87, 8, p. 4159-4167 9 p.

Research output: Contribution to journalArticle

Fractionation
Sorting
Microfluidics
Nanocrystals
Throughput
8 Citations (Scopus)

Microfluidic sorting of protein nanocrystals by size for X-ray free-electron laser diffraction

Abdallah, B. G., Zatsepin, N., Roy-Chowdhury, S., Coe, J., Conrad, C. E., Dörner, K., Sierra, R. G., Stevenson, H. P., Camacho-Alanis, F., Grant, T. D., Nelson, G., James, D., Calero, G., Wachter, R., Spence, J., Weierstall, U., Fromme, P. & Ros, A., Jul 1 2015, In : Structural Dynamics. 2, 4, 041719.

Research output: Contribution to journalArticle

X ray lasers
Free electron lasers
classifying
Sorting
Microfluidics
3 Citations (Scopus)

NMR Structure of Francisella tularensis Virulence Determinant Reveals Structural Homology to Bet v1 Allergen Proteins

Zook, J., Mo, G., Sisco, N. J., Craciunescu, F. M., Hansen, D., Baravati, B., Cherry, B., Sykes, K., Wachter, R., Van Horn, W. & Fromme, P., Jun 3 2015, In : Structure. 23, 6, p. 1116-1122 7 p., 3165.

Research output: Contribution to journalArticle

Francisella tularensis
Allergens
Lipoproteins
Virulence
Magnetic Resonance Spectroscopy
2 Citations (Scopus)

Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum

Yang, J. H., Sarrou, I., Martin-Garcia, J. M., Zhang, S., Redding, K. & Fromme, P., Jun 8 2015, In : Protein Expression and Purification. 114, p. 1-8 8 p.

Research output: Contribution to journalArticle

Adenosine Triphosphate
Detergents
Adenosine Triphosphatases
Gels
Holoenzymes
33 Citations (Scopus)

Serial femtosecond crystallography of soluble proteins in lipidic cubic phase

Fromme, R., Ishchenko, A., Metz, M., Chowdhury, S. R., Basu, S., Boutet, S., Fromme, P., White, T. A., Barty, A., Spence, J., Weierstall, U., Liu, W. & Cherezov, V., Sep 1 2015, In : IUCrJ. 2, p. 545-551 7 p.

Research output: Contribution to journalArticle

Crystallography
crystallography
Microcrystals
proteins
Proteins
7 Citations (Scopus)

Serial femtosecond X-ray diffraction of enveloped virus microcrystals

Lawrence, R. M., Conrad, C. E., Zatsepin, N., Grant, T. D., Liu, H., James, D., Nelson, G., Subramanian, G., Aquila, A., Hunter, M. S., Liang, M., Boutet, S., Coe, J., Spence, J., Weierstall, U., Liu, W., Fromme, P., Cherezov, V. & Hogue, B., Jul 1 2015, In : Structural Dynamics. 2, 4, 041720.

Research output: Contribution to journalArticle

Microcrystals
microcrystals
viruses
Viruses
X ray diffraction