John Spence

Regents Professor

  • 17516 Citations
  • 64 h-Index
1900 …2022

Research output per year

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Research Output

2015

Image reconstruction in serial femtosecond nanocrystallography using x-ray free-electron lasers

Chen, J. P. J., Kirian, R., Beyerlein, K. R., Bean, R. J., Morgan, A. J., Yefanov, O. M., Arnal, R. D., Wojtas, D. H., Bones, P. J., Chapman, H. N., Spence, J. & Millane, R. P., 2015, Proceedings of SPIE - The International Society for Optical Engineering. SPIE, Vol. 9600. 960002

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation

Nogly, P., James, D., Wang, D., White, T. A., Zatsepin, N., Shilova, A., Nelson, G., Liu, H., Johansson, L., Heymann, M., Jaeger, K., Metz, M., Wickstrand, C., Wu, W., Båth, P., Berntsen, P., Oberthuer, D., Panneels, V., Cherezov, V., Chapman, H. & 7 others, Schertler, G., Neutze, R., Spence, J., Moraes, I., Burghammer, M., Standfuss, J. & Weierstall, U., Feb 26 2015, In : IUCrJ. 2, p. 168-176 9 p.

Research output: Contribution to journalArticle

103 Scopus citations
10 Scopus citations

Serial femtosecond crystallography of soluble proteins in lipidic cubic phase

Fromme, R., Ishchenko, A., Metz, M., Chowdhury, S. R., Basu, S., Boutet, S., Fromme, P., White, T. A., Barty, A., Spence, J., Weierstall, U., Liu, W. & Cherezov, V., Sep 1 2015, In : IUCrJ. 2, p. 545-551 7 p.

Research output: Contribution to journalArticle

36 Scopus citations

Serial femtosecond X-ray diffraction of enveloped virus microcrystals

Lawrence, R. M., Conrad, C. E., Zatsepin, N., Grant, T. D., Liu, H., James, D., Nelson, G., Subramanian, G., Aquila, A., Hunter, M. S., Liang, M., Boutet, S., Coe, J., Spence, J., Weierstall, U., Liu, W., Fromme, P., Cherezov, V. & Hogue, B., Jul 1 2015, In : Structural Dynamics. 2, 4, 041720.

Research output: Contribution to journalArticle

9 Scopus citations

Structural basis for bifunctional peptide recognition at human δ-opioid receptor

Fenalti, G., Zatsepin, N., Betti, C., Giguere, P., Han, G. W., Ishchenko, A., Liu, W., Guillemyn, K., Zhang, H., James, D., Wang, D., Weierstall, U., Spence, J., Boutet, S., Messerschmidt, M., Williams, G. J., Gati, C., Yefanov, O. M., White, T. A., Oberthuer, D. & 16 others, Metz, M., Yoon, C. H., Barty, A., Chapman, H. N., Basu, S., Coe, J., Conrad, C. E., Fromme, R., Fromme, P., Tourwé, D., Schiller, P. W., Roth, B. L., Ballet, S., Katritch, V., Stevens, R. C. & Cherezov, V., Mar 6 2015, In : Nature Structural and Molecular Biology. 22, 3, p. 265-268 4 p.

Research output: Contribution to journalArticle

97 Scopus citations

The linac coherent light source single particle imaging road map

Aquila, A., Barty, A., Bostedt, C., Boutet, S., Carini, G., DePonte, D., Drell, P., Doniach, S., Downing, K. H., Earnest, T., Elmlund, H., Elser, V., Gühr, M., Hajdu, J., Hastings, J., Hau-Riege, S. P., Huang, Z., Lattman, E. E., Maia, F. R. N. C., Marchesini, S. & 10 others, Ourmazd, A., Pellegrini, C., Santra, R., Schlichting, I., Schroer, C., Spence, J., Vartanyants, I. A., Wakatsuki, S., Weis, W. I. & Williams, G. J., Jul 1 2015, In : Structural Dynamics. 2, 4, 041701.

Research output: Contribution to journalArticle

91 Scopus citations

X-ray lasers and serial crystallography

Spence, J., Apr 30 2015, In : IUCrJ. 2, p. 305-306 2 p.

Research output: Contribution to journalArticle

5 Scopus citations
2014
29 Scopus citations
15 Scopus citations

Direct phasing in femtosecond nanocrystallography. I. Diffraction characteristics

Chen, J. P. J., Spence, J. & Millane, R. P., 2014, In : Acta Crystallographica Section A: Foundations and Advances. 70, 2, p. 143-153 11 p.

Research output: Contribution to journalArticle

13 Scopus citations

Double-focusing mixing jet for XFEL study of chemical kinetics

Wang, D., Weierstall, U., Pollack, L. & Spence, J., Nov 1 2014, In : Journal of Synchrotron Radiation. 21, p. 1364-1366 3 p.

Research output: Contribution to journalArticle

45 Scopus citations

Expression, purification and crystallization of CTB-MPR, a candidate mucosal vaccine component against HIV-1

Lee, H. H., Cherni, I., Yu, H., Fromme, R., Doran, J. D., Grotjohann, I., Mittman, M., Basu, S., Deb, A., Dörner, K., Aquila, A., Barty, A., Boutet, S., Chapman, H. N., Doak, R. B., Hunter, M. S., James, D., Kirian, R., Kupitz, C., Lawrence, R. M. & 17 others, Liu, H., Nass, K., Schlichting, I., Schmidt, K., Seibert, M. M., Shoeman, R. L., Spence, J., Stellato, F., Weierstall, U., Williams, G. J., Yoon, C., Wang, D., Zatsepin, N., Hogue, B., Matoba, N., Fromme, P. & Leket-Mor, T., Aug 29 2014, In : IUCrJ. 1, p. 305-317 13 p.

Research output: Contribution to journalArticle

5 Scopus citations

Femtosecond X-ray diffraction from two-dimensional protein crystals

Frank, M., Carlson, D. B., Hunter, M. S., Williams, G. J., Messerschmidt, M., Zatsepin, N., Barty, A., Benner, W. H., Chu, K., Graf, A. T., Hau-Riege, S. P., Kirian, R., Padeste, C., Pardini, T., Pedrini, B., Segelke, B., Seibert, M. M., Spence, J., Tsai, C. J., Lane, S. M. & 5 others, Li, X. D., Schertler, G., Boutet, S., Coleman, M. & Evans, J. E., Feb 28 2014, In : IUCrJ. 1, p. 95-100 6 p.

Research output: Contribution to journalArticle

54 Scopus citations

Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

Weierstall, U., James, D., Wang, C., White, T. A., Wang, D., Liu, W., Spence, J., Bruce Doak, R., Nelson, G., Fromme, P., Fromme, R., Grotjohann, I., Kupitz, C., Zatsepin, N., Liu, H., Basu, S., Wacker, D., Won Han, G., Katritch, V., Boutet, S. & 17 others, Messerschmidt, M., Williams, G. J., Koglin, J. E., Marvin Seibert, M., Klinker, M., Gati, C., Shoeman, R. L., Barty, A., Chapman, H. N., Kirian, R., Beyerlein, K. R., Stevens, R. C., Li, D., Shah, S. T. A., Howe, N., Caffrey, M. & Cherezov, V., Feb 14 2014, In : Nature Communications. 5, 3309.

Research output: Contribution to journalArticle

307 Scopus citations

Near edge fine structure analysis of copper in Cu-Bi<inf>2</inf>Se<inf>3</inf> topological insulators

Subramanian, G., Jiang, N., Chen, Y. & Spence, J., Aug 1 2014, In : Microscopy and Microanalysis. 20, 3, p. 160-161 2 p.

Research output: Contribution to journalArticle

1 Scopus citations

Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser

Kupitz, C., Basu, S., Grotjohann, I., Fromme, R., Zatsepin, N., Rendek, K. N., Hunter, M. S., Shoeman, R. L., White, T. A., Wang, D., James, D., Yang, J. H., Cobb, D. E., Reeder, B., Sierra, R. G., Liu, H., Barty, A., Aquila, A. L., Deponte, D., Kirian, R. & 39 others, Bari, S., Bergkamp, J. J., Beyerlein, K. R., Bogan, M. J., Caleman, C., Chao, T. C., Conrad, C. E., Davis, K. M., Fleckenstein, H., Galli, L., Hau-Riege, S. P., Kassemeyer, S., Laksmono, H., Liang, M., Lomb, L., Marchesini, S., Martin, A. V., Messerschmidt, M., Milathianaki, D., Nass, K., Ros, A., Roy-Chowdhury, S., Schmidt, K., Seibert, M., Steinbrener, J., Stellato, F., Yan, L., Yoon, C., Moore, T., Moore, A., Pushkar, Y., Williams, G. J., Boutet, S., Doak, R. B., Weierstall, U., Frank, M., Chapman, H. N., Spence, J. & Fromme, P., Sep 11 2014, In : Nature. 513, 7517, p. 261-265 5 p.

Research output: Contribution to journalArticle

272 Scopus citations

Solving protein nanocrystals by cryo-EM: Multiple scattering artifacts

Subramanian, G., Basu, S., Liu, H., Zuo, J. M. & Spence, J., Aug 1 2014, In : Microscopy and Microanalysis. 20, 3, p. 1242-1243 2 p.

Research output: Contribution to journalArticle

The birth of a new field

Spence, J. & Chapman, H. N., Jul 17 2014, In : Philosophical Transactions of the Royal Society B: Biological Sciences. 369, 1647, 20130309.

Research output: Contribution to journalArticle

23 Scopus citations

The indexing ambiguity in serial femtosecond crystallography (SFX) resolved using an expectation maximization algorithm

Liu, H. & Spence, J., Oct 10 2014, In : IUCrJ. 1, p. 393-401 9 p.

Research output: Contribution to journalArticle

18 Scopus citations

Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein

Tenboer, J., Basu, S., Zatsepin, N., Pande, K., Milathianaki, D., Frank, M., Hunter, M., Boutet, S., Williams, G. J., Koglin, J. E., Oberthuer, D., Heymann, M., Kupitz, C., Conrad, C., Coe, J., Roy-Chowdhury, S., Weierstall, U., James, D., Wang, D., Grant, T. & 16 others, Barty, A., Yefanov, O., Scales, J., Gati, C., Seuring, C., Srajer, V., Henning, R., Schwander, P., Fromme, R., Ourmazd, A., Moffat, K., Van Thor, J. J., Spence, J., Fromme, P., Chapman, H. N. & Schmidt, M., Dec 5 2014, In : Science. 346, 6214, p. 1242-1246 5 p.

Research output: Contribution to journalArticle

228 Scopus citations

Time-resolved spectra from millivolt EELS data

Li, C., Subramanian, G. & Spence, J., 2014, In : Microscopy and Microanalysis. 20, 3, p. 837-846 10 p.

Research output: Contribution to journalArticle

2 Scopus citations

Trace phase detection and strain characterization from serial X-ray free-electron laser crystallography of a Pr<inf>0.5</inf>Ca<inf>0.5</inf>MnO<inf>3</inf> powder

Beyerlein, K. R., Jooss, C., Barty, A., Bean, R., Boutet, S., Dhesi, S. S., Doak, R. B., Först, M., Galli, L., Kirian, R., Kozak, J., Lang, M., Mankowsky, R., Messerschmidt, M., Spence, J., Wang, D., Weierstall, U., White, T. A., Williams, G. J., Yefanov, O. & 3 others, Zatsepin, N., Cavalleri, A. & Chapman, H. N., Mar 3 2014, In : Powder Diffraction. 30, p. S25-S30

Research output: Contribution to journalArticle

3 Scopus citations

Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser

Arnlund, D., Johansson, L. C., Wickstrand, C., Barty, A., Williams, G. J., Malmerberg, E., Davidsson, J., Milathianaki, D., DePonte, D. P., Shoeman, R. L., Wang, D., James, D., Katona, G., Westenhoff, S., White, T. A., Aquila, A., Bari, S., Berntsen, P., Bogan, M., Van Driel, T. B. & 26 others, Doak, R. B., Kjær, K. S., Frank, M., Fromme, R., Grotjohann, I., Henning, R., Hunter, M. S., Kirian, R., Kosheleva, I., Kupitz, C., Liang, M., Martin, A. V., Nielsen, M. M., Messerschmidt, M., Seibert, M. M., Sjöhamn, J., Stellato, F., Weierstall, U., Zatsepin, N., Spence, J., Fromme, P., Schlichting, I., Boutet, S., Groenhof, G., Chapman, H. N. & Neutze, R., 2014, In : Nature Methods. 11, 9, p. 923-926 4 p.

Research output: Contribution to journalArticle

108 Scopus citations
161 Scopus citations

X-ray lasers and crystallography

Spence, J., Apr 30 2014, In : IUCrJ. 1, p. 151-152 2 p.

Research output: Contribution to journalArticle

1 Scopus citations
2013

Casimir torque between birefringent plates with metamaterials

Chen, X. & Spence, J., Sep 2013, In : Physica Status Solidi (A) Applications and Materials Science. 210, 9, p. 1925-1932 8 p.

Research output: Contribution to journalArticle

4 Scopus citations

Development of coherent scattering and diffractive imaging and the COSMIC facility at the Advanced Light Source

Shapiro, D., Roy, S., Celestre, R., Chao, W., Doering, D., Howells, M., Kevan, S., Kilcoyne, D., Kirz, J., Marchesini, S., Seu, K. A., Schirotzek, A., Spence, J., Tyliszczak, T., Warwick, T., Voronov, D. & Padmore, H. A., 2013, In : Journal of Physics: Conference Series. 425, PART 19, 192011.

Research output: Contribution to journalArticle

6 Scopus citations

Natively inhibited trypanosoma brucei cathepsin B structure determined by using an x-ray laser

Redecke, L., Nass, K., DePonte, D. P., White, T. A., Rehders, D., Barty, A., Stellato, F., Liang, M., Barends, T. R. M., Boutet, S., Williams, G. J., Messerschmidt, M., Seibert, M. M., Aquila, A., Arnlund, D., Bajt, S., Barth, T. B., Bogan, M. J., Caleman, C., Chao, T. C. & 29 others, Doak, R. B., Fleckenstein, H., Frank, M., Fromme, R., Galli, L., Grotjohann, I., Hunter, M. S., Johansson, L. C., Kassemeyer, S., Katona, G., Kirian, R., Koopmann, R., Kupitz, C., Lomb, L., Martin, A. V., Mogk, S., Neutze, R., Shoeman, R. L., Steinbrener, J., Timneanu, N., Wang, D., Weierstall, U., Zatsepin, N., Spence, J., Fromme, P., Schlichting, I., Duszenko, M., Betzel, C. & Chapman, H. N., Jan 11 2013, In : Science. 339, 6116, p. 227-230 4 p.

Research output: Contribution to journalArticle

302 Scopus citations

Serial femtosecond crystallography of G protein-coupled receptors

Liu, W., Wacker, D., Gati, C., Han, G. W., James, D., Wang, D., Nelson, G., Weierstall, U., Katritch, V., Barty, A., Zatsepin, N., Li, D., Messerschmidt, M., Boutet, S., Williams, G. J., Koglin, J. E., Seibert, M. M., Wang, C., Shah, S. T. A., Basu, S. & 13 others, Fromme, R., Kupitz, C., Rendek, K. N., Grotjohann, I., Fromme, P., Kirian, R., Beyerlein, K. R., White, T. A., Chapman, H. N., Caffrey, M., Spence, J., Stevens, R. C. & Cherezov, V., 2013, In : Science. 342, 6165, p. 1521-1524 4 p.

Research output: Contribution to journalArticle

286 Scopus citations

Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Johansson, L. C., Arnlund, D., Katona, G., White, T. A., Barty, A., Deponte, D. P., Shoeman, R. L., Wickstrand, C., Sharma, A., Williams, G. J., Aquila, A., Bogan, M. J., Caleman, C., Davidsson, J., Doak, R. B., Frank, M., Fromme, R., Galli, L., Grotjohann, I., Hunter, M. S. & 25 others, Kassemeyer, S., Kirian, R., Kupitz, C., Liang, M., Lomb, L., Malmerberg, E., Martin, A. V., Messerschmidt, M., Nass, K., Redecke, L., Seibert, M. M., Sjöhamn, J., Steinbrener, J., Stellato, F., Wang, D., Wahlgren, W. Y., Weierstall, U., Westenhoff, S., Zatsepin, N., Boutet, S., Spence, J., Schlichting, I., Chapman, H. N., Fromme, P. & Neutze, R., Dec 19 2013, In : Nature Communications. 4, 2911.

Research output: Contribution to journalArticle

65 Scopus citations

The extraction of single-particle diffraction patterns from a multiple-particle diffraction pattern

Martin, A. V., Morgan, A. J., Ekeberg, T., Loh, N. D., Maia, F. R. N. C., Wang, F., Spence, J. & Chapman, H. N., Jul 1 2013, In : Optics Express. 21, 13, p. 15102-15112 11 p.

Research output: Contribution to journalArticle

3 Scopus citations

Three-dimensional single-particle imaging using angular correlations from X-ray laser data

Liu, H., Poon, B. K., Saldin, D. K., Spence, J. & Zwart, P. H., Jul 2013, In : Acta Crystallographica Section A: Foundations of Crystallography. 69, 4, a69.

Research output: Contribution to journalArticle

30 Scopus citations
8 Scopus citations
2012

Femtosecond free-electron laser X-ray diffraction data sets for algorithm development

Kassemeyer, S., Steinbrener, J., Lomb, L., Hartmann, E., Aquila, A., Barty, A., Martin, A. V., Hampton, C. Y., Bajt, S., Barthelmess, M., Barends, T. R. M., Bostedt, C., Bott, M., Bozek, J. D., Coppola, N., Cryle, M., De Ponte, D. P., Doak, R. B., Epp, S. W., Erk, B. & 42 others, Fleckenstein, H., Foucar, L., Graafsma, H., Gumprecht, L., Hartmann, A., Hartmann, R., Hauser, G., Hirsemann, H., Hömke, A., Holl, P., Jönsson, O., Kimmel, N., Krasniqi, F., Liang, M., Maia, F. R. N. C., Marchesini, S., Nass, K., Reich, C., Rolles, D., Rudek, B., Rudenko, A., Schmidt, C., Schulz, J., Shoeman, R. L., Sierra, R. G., Soltau, H., Spence, J., Starodub, D., Stellato, F., Stern, S., Stier, G., Svenda, M., Weidenspointner, G., Weierstall, U., White, T. A., Wunderer, C., Frank, M., Chapman, H. N., Ullrich, J., Strüder, L., Bogan, M. J. & Schlichting, I., Feb 13 2012, In : Optics Express. 20, 4, p. 4149-4158 10 p.

Research output: Contribution to journalArticle

53 Scopus citations

Injector for scattering measurements on fully solvated biospecies

Weierstall, U., Spence, J. & Doak, R. B., Mar 2012, In : Review of Scientific Instruments. 83, 3, 035108.

Research output: Contribution to journalArticle

148 Scopus citations

Lipidic phase membrane protein serial femtosecond crystallography

Johansson, L. C., Arnlund, D., White, T. A., Katona, G., Deponte, D. P., Weierstall, U., Doak, R. B., Shoeman, R. L., Lomb, L., Malmerberg, E., Davidsson, J., Nass, K., Liang, M., Andreasson, J., Aquila, A., Bajt, S., Barthelmess, M., Barty, A., Bogan, M. J., Bostedt, C. & 48 others, Bozek, J. D., Caleman, C., Coffee, R., Coppola, N., Ekeberg, T., Epp, S. W., Erk, B., Fleckenstein, H., Foucar, L., Graafsma, H., Gumprecht, L., Hajdu, J., Hampton, C. Y., Hartmann, R., Hartmann, A., Hauser, G., Hirsemann, H., Holl, P., Hunter, M. S., Kassemeyer, S., Kimmel, N., Kirian, R., Maia, F. R. N. C., Marchesini, S., Martin, A. V., Reich, C., Rolles, D., Rudek, B., Rudenko, A., Schlichting, I., Schulz, J., Seibert, M. M., Sierra, R. G., Soltau, H., Starodub, D., Stellato, F., Stern, S., Strülder, L., Timneanu, N., Ullrich, J., Wahlgren, W. Y., Wang, X., Weidenspointner, G., Wunderer, C., Fromme, P., Chapman, H. N., Spence, J. & Neutze, R., Mar 2012, In : Nature Methods. 9, 3, p. 263-265 3 p.

Research output: Contribution to journalArticle

121 Scopus citations

Microscopic linear liquid streams in vacuum: Injection of solvated biological samples into X-ray free electron lasers

Doak, R. B., Deponte, D. P., Nelson, G., Camacho-Alanis, F., Ros, A., Spence, J. & Weierstall, U., 2012, AIP Conference Proceedings. 1 ed. Vol. 1501. p. 1314-1323 10 p.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations

On the dose-rate threshold of beam damage in TEM

Jiang, N. & Spence, J., Feb 2012, In : Ultramicroscopy. 113, p. 77-82 6 p.

Research output: Contribution to journalArticle

48 Scopus citations