MOESM6 of Characterization of lysosomal proteins Progranulin and Prosaposin and their interactions in Alzheimer’s disease and aged brains: increased levels correlate with neuropathology

Additional file 6 Figure S6 Complete western blot images of immunoprecipitated brain and cell samples to identify presence of granulin or saposin peptides. A) Complete western blot image of brain samples immunoprecipitated with PGRN antibody conjugated magnetic beads and detected with antibody to PSAP. The blots showed no evidence of lower molecular weight saposin peptides. B) Complete western blot image of brain samples immunoprecipitated with PGRN antibody conjugated magnetic beads and detected with antibody to PGRN. The blots showed no evidence of lower molecular weight granulin peptides. C) Complete western blot images of PGRN-overexpressing HEK and brain samples (AD) immunoprecipitated with magnetic beads conjugated with control normal goat immunoglobulin (goat IgG), or PGRN or PSAP antibodies and probed with antibody to PGRN. Image shows that granulin peptides could be minimally detected only in HEK cells expressing high levels of PGRN protein. Blot also shows total extracts from SH-SY5Y differentiated neurons (SY) and AD brain analyzed in parallel showing no detectable granulin peptides.