MOESM4 of Characterization of lysosomal proteins Progranulin and Prosaposin and their interactions in Alzheimer’s disease and aged brains: increased levels correlate with neuropathology

Additional file 4 Figure S4. Complete western blot images of MTG protein samples. A). Complete western blot images of MTG brain sauremples probed with antibody to detect PGRN polypeptides in Low plaque (LP), high plaque (HP) and AD samples. Major PGRN polypeptide bands detected at approximately 75–80 kDa, with less intense bands at 55 kDa. These blots showed absence of low molecular weight granulin peptides. Top Image: Shorter exposure of western blot. Lower Image: Longer exposure of western blot to demonstrate if granulin peptides were present. B). Complete western blot images of MTG brain samples probed with antibody to detect PSAP polypeptides in Low plaque (LP), high plaque (HP) and AD samples. Major PSAP polypeptide bands detected at approximately 72 kDa. Additional polypeptide bands are detectable with image enhancement indicating detection of PSAP-derived saposin peptides. Top Image: Shorter exposure of western blot. Lower image: Longer exposure of western blot images of MTG brain samples probed with antibody to detect PSAP polypeptides. Bands with molecular weights of saposin peptides are indicated. These were noticeable in AD samples.