MOESM3 of Characterization of lysosomal proteins Progranulin and Prosaposin and their interactions in Alzheimer’s disease and aged brains: increased levels correlate with neuropathology

Additional file 3 Figure S3 Limited colocalization of PGRN with markers defining neurites and neuritic plaques. A-C): Confocal image of PGRN (green)(A) immunoreactivity associated with a neuritic plaque identified with pTau antibody AT180 (B) with merged image (C) in an AD case showing no colocalization of staining. DAPI staining identified nuclei and also highly aggregated amyloid within plaques. Scale bar represents 20 μm. D-F): Confocal image of PGRN (green)(D) immunoreactivity associated with neurites and neuritic plaque identified with pan-neurofilament antibody SMI312 (E) with merged DAPI-stained image (F) in an AD case showing limited colocalization of staining. Scale bar represents 20 μm. G-I): Confocal image of PGRN (green)(G) immunoreactivity associated with neurites and neuritic plaque identified with synaptophysin antibody (H) with merged DAPI-stained image (I) in an AD case showing no colocalization of staining. Scale bar represents 20 μm. These images were acquired using a Leica SP8 confocal microscope.