In-solution Y-chromosome capture-enrichment on ancient DNA libraries

  • Alexandra Sockell (Contributor)
  • María C. Ávila-Arcos (Contributor)
  • Diana I. Cruz-Dávalos (Contributor)
  • G. David Poznik (Contributor)
  • Maria A. Nieves-Colón (Contributor)
  • Hannes Schroeder (Contributor)
  • Carlos D. Bustamante (Contributor)
  • Anna Sapfo Malaspinas (Contributor)
  • Anne Stone (Contributor)

Dataset

Description

Abstract Background As most ancient biological samples have low levels of endogenous DNA, it is advantageous to enrich for specific genomic regions prior to sequencing. One approach—in-solution capture-enrichment—retrieves sequences of interest and reduces the fraction of microbial DNA. In this work, we implement a capture-enrichment approach targeting informative regions of the Y chromosome in six human archaeological remains excavated in the Caribbean and dated between 200 and 3000 years BP. We compare the recovery rate of Y-chromosome capture (YCC) alone, whole-genome capture followed by YCC (WGC + YCC) versus non-enriched (pre-capture) libraries. Results The six samples show different levels of initial endogenous content, with very low (
Date made availableAug 14 2018
Publisherfigshare Academic Research System

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