Additional file 8: Figure S8. Characterization of endogeneous RBM45 CRISPR-cas9 edited HEK293 cells. CRISPR-cas9 genome editing was used to generate HEK293 cells expressing N-terminally 2x FLAG-tagged RBM45. (a) RBM45 transcript levels were evaluated by real-time PCR. The barplot presents the mean RBM45 transcript levels relative to unedited cells expressing wild-type RBM45. (b) Immunofluorescence was performed using an antibody to RBM45 in CRISPR-edited HEK293 cells. The results show that the abundance and subcellular distribution of RBM45 protein and HEK293 cell nuclear morphology are not altered by the 2X FLAG tag.
|Date made available||2020|