Additional file 6: Figure S6. Subcellular localization of full-length, domain deletion, and mutant NLS RBM45 constructs in untreated cells. (a) Schematic showing functional domains and their position in the full-length RBM45 protein. RRM = RNA recognition motif, HOA = homo-oligomerization domain, NLS = nuclear localization sequence. HEK293 cells were transfected with constructs encoding N-terminally HA-tagged wild-type (WT) or domain-modified forms of RBM45 as indicated. (b) In untreated cells, the distribution of WT RBM45 is diffuse and nuclear. (c) Removal of the RBM45 NLS (∆NLS) leads to cytoplasmic sequestration of RBM45. (d-g) Removal of RRM1 (∆RRM1; d), RRM2 (∆RRM2; e), RRM3 (∆RRM3; f) and the HOA domain (∆HOA; g) do not alter the diffuse, nuclear localization of RBM45. No HSF1-positive NSBs are observed in untreated cells expressing any of the indicated RBM45 constructs. For all images, scale bar = 5 μm.
|Date made available||2020|