Additional file 4: Figure S4. siRNA targeting of RBM45 and SatIII. HEK293 cells were transfected with the indicated siRNA and transcript levels were measured by real-time PCR. Each bar presents the relative transcript abundance, expressed as a proportion of the corresponding transcript level in untreated cells transfected with a scrambled control siRNA. (a) Evaluation of two unique siRNAs, each targeting RBM45. (b) Evaluation of satellite III (SatIII) knockdown efficiency in untreated and heat shocked (42 °C for 2 h) cells.