PCR detection of mutants with specific primers for each strain. Detection of each specific strain is observed in lane 2 and 4, although unspecific bands are also observed when the primers are exposed to the other strain (lane 3 and 5), which is different in only one nucleotide. (TIFF 1377Â kb)
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Franco, L. S. (Creator), Holechek, S. A. (Creator), Caplan, M. R. (Creator), Blattman, J. N. (Creator) (2017). Additional file 3: of Sequence-specific detection of different strains of LCMV in a single sample using tentacle probes. figshare Academic Research System. 10.6084/m9.figshare.c.3904471_d3.v1